کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9885598 | 1537333 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The p100 EBNA-2 coactivator: a highly conserved protein found in a range of exocrine and endocrine cells and tissues in cattle
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کلمات کلیدی
UTREBNA-2ORFRT-PCRIPTGcDNA - cDNADNA complementary to RNA - DNA مکمل RNAPSI-BLAST - PSI BLASTamino acid - آمینو اسیدPosition-Specific Iterated BLAST - انحراف موضعی خاصBlast - انفجارisopropyl β-D-1-thiogalactopyranoside - ایزوپروپیل β-D-1-thiogalactopyranosideNuclease - تخریبrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدTudor domain - دامنه تودورopen reading frame - قاب خواندن بازRace - مسابقهuntranslated region - منطقه غیر ترجمهNucleotide - نوکلئوتیدpolymerase chain reaction - واکنش زنجیره ای پلیمرازreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسPCR - واکنش زنجیرهٔ پلیمرازPancreas - پانکراسMammary - پستانداران
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The p100 transcriptional coactivator is an evolutionarily conserved protein that has been shown to be a coactivator of the Epstein-Barr virus-encoded transcription factor EBNA-2, as well as Stat5 and Stat6. However, the p100 genomic organisation, phylogeny and expression have not been analysed in detail and its physiological role is uncertain. The cDNA and amino acid sequence of bovine p100 was obtained, and the genomic organisation of the human p100 gene was determined. Homologues of p100 were found in the genomes of 21 diverse eukaryotes. Western blot and immunohistochemical analyses revealed that the bovine p100 protein is present in a range of exocrine and endocrine cells and tissues, including the lactating mammary gland, pancreas, adrenal, parotid, anterior pituitary, corpus luteum, ovarian follicular cells, placenta and small intestine. P100 was present in the nuclei of mammary epithelial cells and pancreatic acinar cells, but only in the extranuclear compartment of the other immunopositive tissues. These data indicate that the p100 protein plays a fundamental role in eukaryotic biology, and functions in secretory cells, at least in cattle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1681, Issues 2â3, 11 January 2005, Pages 126-133
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1681, Issues 2â3, 11 January 2005, Pages 126-133
نویسندگان
Marita K. Broadhurst, Rita S.-F. Lee, Sarah Hawkins, Thomas T. Wheeler,