کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886460 | 1537826 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
GC-rich sequences in the 5-lipoxygenase gene promoter are required for expression in Mono Mac 6 cells, characterization of a novel Sp1 binding site
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کلمات کلیدی
EicosanoidEMSA5-HETEPMNLFPU5-LOPBSINREGR-1TGFβ1,25(OH)2D3 - 1،25 (OH) 2D35-Lipoxygenase - 5-لیپوکسیژنازSp1 - SP1Electrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزArachidonic acid - اسید آراشیدونیکTransforming growth factor β - تبدیل فاکتور رشد βInitiator element - عنصر آغازگرPhosphate buffered saline - فسفات بافر شورLeukotriene - لکوترینpolymorphonuclear leukocytes - لکوسیت های پلی مورفونیک هسته ایVitamin D3 - ویتامین D3، کوله کلسیفرول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
5-lipoxygenase (5LO) catalyzes formation of leukotrienes, mediators with roles in several inflammatory disorders, including atherosclerosis. The human 5LO gene promoter contain multiple GC-boxes. The relevance of these for expression of 5LO in the human monocytic cell line Mono Mac 6 (MM6) was studied. A downregulating effect of the GC-box binding compound mithramycin indicated that GC-rich sequences in the 5LO gene promoter are important for expression of native 5LO. In DNase I footprinting, mithramycin and Sp1 protected known GC-boxes, but also a novel Sp1 binding site was found, comprising 20 bp upstream of the major transcription initiation site, beside an Initiator-like sequence. Mutation of this site reduced Sp1 binding and expression of reporter genes in MM6 cells, compatible with a function as basal promoter element for the TATA-less 5LO gene. When differentiation was induced by TGFβ and vitamin D3, 5LO expression became prominent, but expression levels of Sp1/3 and Egr-1 were the same as for control cells. Also, 5LO reporter gene activity in transiently transfected MM6 cells was insensitive to differentiation. Thus, the GC-rich part of the 5LO gene promoter, including a novel Sp1 site, appear important for basal (rather than upregulated) transcription of 5LO in MM6 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1738, Issues 1â3, 30 December 2005, Pages 37-47
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1738, Issues 1â3, 30 December 2005, Pages 37-47
نویسندگان
David Dishart, Nicole Schnur, Niko Klan, Oliver Werz, Dieter Steinhilber, Bengt Samuelsson, Olof RÃ¥dmark,