کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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99028 | 160918 | 2011 | 5 صفحه PDF | دانلود رایگان |
Traditionally, DNA extracts from biological evidence items have been concentrated and rinsed using microdialysis filtration units, including the Centricon® and Microcon® centrifugal filter devices. As an alternative to microdialysis filtration, we present an optimized method for using NucleoSpin® XS silica columns to concentrate and clean-up aqueous extracts from the organic extraction of DNA from biological samples. The method can be used with standard organic extraction and dithiothreitol (DTT)-based differential extraction methods with no modifications to these methods prior to the concentration and clean-up step. Extracts from laboratory-prepared bloodstains, saliva and semen stains have been successfully amplified with both qPCR and STR assays. Finally, the total time to process a set of samples with the NucleoSpin® XS column is approximately 30 min vs. approximately 1.5 h with the Centricon® YM-100 filter device.
Journal: Forensic Science International: Genetics - Volume 5, Issue 3, June 2011, Pages 226–230