کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9905089 | 1546514 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Exfoliated buccal and microdissected lung cell expression of antioxidant enzymes
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کلمات کلیدی
Plasma nicotineGenetic susceptibility - استعداد ژنتیکی یا آسیب پذیری ژنتیکی Gene expression - بیان ژنDiet - رژیم غذاییexfoliated cells - سلول های خالیSmokers - سیگاری هاAntioxidant metabolism - متابولیسم آنتیاکسیدانBiomarkers - نشانگر زیستی یا بیومارکرNever smokers - هرگز سیگار نکشیدCatalase - کاتالازPlasma cotinine - کتینین پلاسماglutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Exfoliated buccal and microdissected lung cell expression of antioxidant enzymes Exfoliated buccal and microdissected lung cell expression of antioxidant enzymes](/preview/png/9905089.png)
چکیده انگلیسی
Introduction: An exfoliated buccal cell biomarker assay for antioxidant gene transcript levels was used to measure inter-tissue concordance with lung, and inter-subject variability in a lung cancer case-control study. Methods: First, qualitative RNA-specific RT-PCR was used to compare expression in exfoliated buccal cells with that in laser microdissected lung tissue remote from the tumor from 14 individuals providing both specimens. Results: There was complete [100% for quinone oxidoreductase 1 (NQO1), glutathione peroxidase (GPX), and superoxide dismutase 1 (SOD1)], or predominant [85.7% for catalase (CAT)] inter-tissue concordance for qualitative expression. Second, quantitative real-time RT-PCR for antioxidant enzyme transcript levels was performed in exfoliated buccal samples from these same 14 individuals, as well as 28 additional individuals providing buccal cells only, for a total of 42 buccal specimens (19 current smokers and 23 ex- or never-smokers), of whom 26 (61.39%) had a new diagnosis of lung cancer. Discussion: Wide inter-individual expression differences for each gene transcript (>101-104-fold) were observed in the exfoliated buccal cells, unrelated to smoking and case-control status. In multivariate analyses, family history of tobacco-related malignancy correlated inversely with buccal NQO1 and CAT mRNA levels (p = 0.003, p < 0.001, respectively). This antioxidant expression trait may relate to family risk of cancer, but is notably unrelated to oxidant challenges inherent in cigarette smoke.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Detection and Prevention - Volume 29, Issue 6, 2005, Pages 552-561
Journal: Cancer Detection and Prevention - Volume 29, Issue 6, 2005, Pages 552-561
نویسندگان
Shalini V. MS, Ritu PhD, Katherine RN, Ann RN, Angela MS, Simon D. MD,