کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9912483 | 1549789 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stepwise mechanical stretching inhibits chondrogenesis through cell-matrix adhesion mediated by integrins in embryonic rat limb-bud mesenchymal cells
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کلمات کلیدی
ECMPMSFPFAERKRT-PCRGAPDHLIMHEPESFITCDMEMPBSMAPK - MAPKDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoEDTA - اتیلن دی آمین تترا استیک اسید ethylene diamine tetra-acetic acid - اتیلن دیامین تترا استیک اسیدSodium dodecylsulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل سدیم دودسیل سولفات - پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدstandard deviation - انحراف معیارIntegrin - اینتگرینShear stress - تنش برشیpolyvinylidene difluoride - دی فلوئورید پلی وینیلیدینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتPhenylmethylsulfonylfluoride - فنیل متیل سولفونیل فلوئوریدExtracellular matrix - ماتریکس خارج سلولیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHematoxylin and Eosin - هماتوکسیلین و ائوزینReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایparaformaldehyde - پارافرمالدهیدStretch - کش آمدنChondrogenesis - کندروژنیکextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Stepwise mechanical stretching inhibits chondrogenesis through cell-matrix adhesion mediated by integrins in embryonic rat limb-bud mesenchymal cells Stepwise mechanical stretching inhibits chondrogenesis through cell-matrix adhesion mediated by integrins in embryonic rat limb-bud mesenchymal cells](/preview/png/9912483.png)
چکیده انگلیسی
Biomechanical forces are major epigenetic factors that determine the form and differentiation of skeletal tissues, and may be transduced through cell adhesion to the intracellular biochemical signaling pathway. To test the hypothesis that stepwise stretching is translated to molecular signals during early chondrogenesis, we developed a culture system to study the proliferation and differentiation of chondrocytes. Rat embryonic day-12 limb buds were microdissected and dissociated into cells, which were then micromass cultured on a silicone membrane and maintained for up to 7 days. Stepwise-increased stretching was applied to the silicone membrane, which exerted shearing stress on the cultures on day 4 after the initiation of chondrogenesis. Under stretched conditions, type II collagen expression was significantly inhibited by 44% on day 1 and by 67% on day 2, and this difference in type II collagen reached 80% after 3 days of culture. Accumulation of type II collagen protein and the size of the chondrogenic nodules had decreased by 50% on day 3. On the other hand, expression of the non-chondrogenic marker fibronectin was significantly up-regulated by 1.8-fold on day 3, while the up-regulation of type I collagen was minimal, even by day 3. The down-regulation in the expression of chondrogenic markers was completely recovered when cell-extracellular matrix attachment was inhibited by Gly-Arg-Gly-Asp-Ser-Pro-Lys peptide or by the application of blocking antibodies for α2, α5 or β1 integrins. We conclude that shearing stress generated by stepwise stretching inhibits chondrogenesis through integrins, and propose that signal transduction from biomechanical stimuli may be mediated by cell-extracellular matrix adhesion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 84, Issue 1, 31 January 2005, Pages 45-58
Journal: European Journal of Cell Biology - Volume 84, Issue 1, 31 January 2005, Pages 45-58
نویسندگان
Kazuyuki Onodera, Ichiro Takahashi, Yasuyuki Sasano, Jin-Wan Bae, Hidetoshi Mitani, Manabu Kagayama, Hideo Mitani,