A fluorescent microscopy-screening test for efficient STR-typing of telogen hair roots
Keywords: Forensic hair analysis; DAPI; Fluorescence microscopy; Short-tandem-repeat
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Morphological assessment of apoptosis
Keywords: Morphology; H&E; Dapi; Blebbing; Chromatin condensation; TEM; SEM;
Serial culture of murine primary airway epithelial cells and ex vivo replication of human rhinoviruses
Keywords: HRV; human rhinovirus; COPD; chronic obstructive pulmonary disease; LDLr; low density lipoprotein receptor; DMEM; Dulbecco's minimal essential medium; F12; Ham's F12 medium; MEM; minimal essential medium; FCS; fetal calf serum; PCT; progenitor cell target
Comparison of various staining methods for the detection of Cryptosporidium in cell-free culture
Keywords: Cryptosporidium; Cell-free; Staining methods; Sporo-Glo™; Crypto Cel; Fluorescent in-situ hybridisation (FISH); Texas Red; CFSE; DAPI
Detection of inhibition of antimicrobial activity by mycobacterial lysates in human monocytes infected with Legionella pneumophila
Keywords: BCYE; buffered charcoal yeast lysate; ATTC; American Type Culture Collection; CFU; colony forming units; CECT; Spanish Type Culture Collection; DAPI; 4,6,-DiAmidino-2-PhenylIndole; IFN-γ; interferon gamma; IL; interleukin; OADC; oleic acid-bovine albumin
Defective killing of Staphylococcus aureus in atopic dermatitis is associated with reduced mobilization of human β-defensin-3
Keywords: Antimicrobial peptides; defensins; human; bacterial infections; skin; keratinocytes; AD; Atopic dermatitis; DAPI; 4â²-6-Diamidino-2-phenylindole, dihydrochloride; HBD-3; Human β-defensin-3; KGM; Keratinocyte Growth Medium; MRSA; Methicillin-resistant St
Interaction of epithelial cell membrane rafts with Paracoccidioides brasiliensis leads to fungal adhesion and Src-family kinase activation
Keywords: Membrane microdomains; Paracoccidioides brasiliensis; Host-pathogen interactions; Adhesions; Src-Family Kinases; ERK MAP kinases; Brij®98; polyoxyethylene(20) oleyl ether; BSA; bovine serum albumin; CTB; cholera toxin subunit B; DAPI; 2-(4-amidinophenyl)
Chromatin extrusion in resting encystment of Colpoda cucullus: A possible involvement of apoptosis-like nuclear death
Keywords: Colpoda cucullus; Resting encystment; Chromatin extrusion; Apoptosis; Nuclear death; Macronuclear DNA; CE; chromatin extrusion; DAPI; 4â²,6-diamidino-2-phenylindole; AO; Acridine orange; HO; Hoechst 33342;
Brief cross-linking of Fas/APO-1 (CD95) triggers engulfment of pre-apoptotic target cells
Keywords: CTL; cytotoxic T lymphocytes; DAPI; 4â²-6-diamidino-2-phenylindole; DEVD-AMC; aspartate-glutamate-valine-aspartate-7-amino-4-methyl-coumarin; FBS; fetal bovine serum; FPRL-1; formyl-peptide receptor-like-1; HMDM; human monocyte-derived macrophages;
Overexpression of SPINDLIN1 induces cellular senescence, multinucleation and apoptosis
Keywords: 7-AAD; 7-Aminoactinomycin D; DAPI; 4', 6-diamidino-2-phenylindole; EGFP; Enhanced green fluorescent protein; PBS; Phosphate-buffered saline; SA-β-gal activity; Senescence-associated β-galactosidase.; Apoptosis; Cell death; Senescence; SPINDLIN1;
Intrapallidal metabotropic glutamate receptor activation in a rat model of Parkinson's disease: Behavioral and histological analyses
Keywords: ACPD; 1-aminocyclopentane-1S,3R-dicarboxylic acid; 6-OHDA; 6-hydroxydopamine; DAPI; 4â², 6-diamidino-2-phenylindole, dilactate; EP; entopeduncular nucleus; EPSPs; excitatory postsynaptic potentials; GABA; gamma-aminobutyric acid; GP; globus pallidus; IPS
Cytidine-5-diphosphocholine supplement in early life induces stable increase in dendritic complexity of neurons in the somatosensory cortex of adult rats
Keywords: citicholine; barrel cortex; Golgi-Cox; stroke; plasticity; Sholl analysis; CDP-choline; cytidine-5-diphosphocholine; DAPI; 4â²,6-diamidino-2-phenylindole; DMEM; Dulbecco's minimal essential medium; MAP2; microtubule-associated protein 2; P; postnatal d
Anticancer and antiproliferative activity of natural brassinosteroids
Keywords: BrdU; bromodeoxyuridine; BRs; brassinosteroids; DAPI; 4,6-diamidino-2-phenylindole; DMEM; Dulbecco's modified Eagle's medium; MTT; 3-(4,5-dimethyltiazol-2-yl)-2,5-diphenyltetrazolium bromide; PBS; phosphate-buffered saline; Brassinosteroids; Anticance
Increase in Fru-2,6-P2 levels results in altered cell division in Schizosaccharomyces pombe
Keywords: Fru-2,6-P2; Fructose 2,6-bisphosphate; PF2KFB; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase; MAPK; mitogen-activated protein kinase; PI3K; phosphatidylinositol 3-kinase; ROS; reactive oxygen species; TIGAR; TP53-induced glycolysis and apoptosis re
The role of apoptosis in Listeria monocytogenes neural infection: Listeriolysin O interaction with neuroblastoma Neuro-2a cells
Keywords: Apoptosis; Listeria monocytogenes; Neural; Infection; Listeriolysin O; Interaction; Neuroblastoma Neuro-2a; Cytotoxicity; DAPI; Intranucleosomal DNA
Nuclear translocation of immulectin-3 stimulates hemocyte proliferation
Keywords: BH1; Bcl-2 homology domain 1; BrdU; 5-bromo-2â²-deoxy-uridine; DAPI; 4â²,6-diamidino-2-phenylindole; FBS; fetal bovine serum; FITC; fluorescein isothiocyanate; GFP; green fluorescent protein; IML-3; immulectin-3; LPS; lipopolysaccharide; PAGE; polyacryl
Nuclear calcium signaling by inositol trisphosphate in GH3 pituitary cells
Keywords: [Ca2+]C; cytosolic Ca2+ concentration; [Ca2+]N; nuclear Ca2+ concentration; TRH; thyrotropin-releasing hormone; NE; nuclear envelope; ER; endoplasmic reticulum; IP3; inositol-1,4,5-trisphosphate; AEQ; aequorin; GFP; green fluorescent protein; HSV; herpes
Brain regional and cellular localization of gelatinase activity in rat that have undergone transient middle cerebral artery occlusion
Keywords: GM6001; in situ zymography; reperfusion injury; stroke; matrix metalloproteinases; neuroinflammation; BBB; blood-brain barrier; DAPI; 4â²,6-diamidino-2-phenylindole dihydrochloride; DQ; dye quenched; ECM; extracellular matrix; GM6001; N-[(2R)-2-(hydrox
Capsaicin-induced neuronal death and proliferation of the primary sensory neurons located in the nodose ganglia of adult rats
Keywords: nodose ganglion; capsaicin; neurogenesis; neuronal death; rat; sensory neurons; BrdU; 5â²-bromo-2â²-deoxyuridine; cas-3; cleaved caspase 3; DAPI; 4â²,6-diamidino-2-phenylindole; -IR; -immunoreactivity; NfM; neurofilament-M; NG; nodose ganglion; PGP; pr
Cadmium activates the mitogen-activated protein kinase (MAPK) pathway via induction of reactive oxygen species and inhibition of protein phosphatases 2A and 5
Keywords: AD; Alzheimer's disease; ALS; amyotrophic lateral sclerosis; ASK1; apoptosis signal-regulating kinase 1; Cd; cadmium; CM-H2DCFDA; 5-(and-6)-chloromethyl-2â²,7â²-dichlorodihydrofluorescein diacetate; DAPI; 4â²,6-diamidino-2-phenylindole; DMEM; Dulbecco'
Modulation of cell cycles and apoptosis by apicidin in estrogen receptor (ER)-positive and-negative human breast cancer cells
Keywords: HDAC; histone deacetylase; HATs; histone acetyltransferases; ER; estrogen receptor; TSA; trichostatin A; SAHA; suberoylanilide hydroxamic acid; Rb; retinoblastoma; DMEM; Dulbecco's Modified Eagle Medium; FBS; fetal bovine serum; PVDF; polyvinylidene diflu
Characterization and localization of ORFF gene from the LD1 locus of Leishmania donovani
Keywords: BT1; Biopterin transporter1; DAPI; 4', 6-diamidino-2-phenylindole; FACS; Fluorescence activated cell sorter; FCS; Fetal calf serum; GFP; Green Fluorescent Protein; HYG; Hygromycin; NEO; Neomycin; ORFF; Open reading frame F; ORFG; Open reading frame G; PBS
Deletion of exon 8 increases cisplatin-induced E-cadherin cleavage
Keywords: AEC; anti-E-cadherin antibody; Akt/PKB; Akt/protein kinase B; cl; cleaved; CTL; control; DAPI; 4,6-diamidino-2-phenylindole; DMSO; dimethyl sulfoxide; del 8 E-cadherin, d8; E-cadherin with deletion of exon 8; fl; full length; MDA; untransfected, parental
Cloning and characterization of Arenicola marina peroxiredoxin 6, an annelid two-cysteine peroxiredoxin highly homologous to mammalian one-cysteine peroxiredoxins
Keywords: Bt; Bos taurus; CHP; cumene hydroperoxide; Cp; peroxidatic cysteine; Cr; resolving cysteine; DAPI; 4â²,6-diamidino-2-phenylindole; Dox; oxidized dimer; DTT; dithiothreitol; EDTA; ethylenediaminetetraacetic acid; FITC; fluoresceine isothiocyanate; GPX; gl
Characterization of Fetal and Postnatal Enteric Neuronal Cell Lines With Improvement in Intestinal Neural Function
Keywords: α-SMA; α-smooth muscle actin; BrdU; bromodeoxyuridine; DAPI; 4',6-diamidino-2-phenylindole; EFS; electrical field stimulation; ENS; enteric nervous system; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; GDNF; glial cell line-derived neurotrophic fac
Spatiotemporal patterns of smooth muscle cell changes in ascending aortic dilatation with bicuspid and tricuspid aortic valve stenosis: Focus on cell-matrix signaling
Keywords: 26; 29; 35; AI; apoptotic index; BAV; bicuspid aortic valve; Bmf; Bcl-2-modifying factor; DAPI; 4â²,6-diamidino-2â²-phenylindole; ECM; extracellular matrix; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; MMP; matrix metalloproteinases; mRNA; messenger
STI571/doxorubicin concentration-dependent switch for diverse caspase actions in CML cell line K562
Keywords: Ara-C; cytarabine, cytosine arabinoside; Bcl-XL(B-cell leukemia/lymphoma extra long); anti-apoptotic protein; CML; chronic myelogenous leukemia; DAPI; 4,6-diamidino-2-phenylidole; ÎΨm; mitochondrial transmembrane potential; DMSO; dimethyl sulfoxide; DOX
A dynamic and chamber-specific mitochondrial remodeling in right ventricular hypertrophy can be therapeutically targeted
Keywords: DAPI; 4,6-diamino-2-phenylindole; DCA; dichloroacetate; LV; left ventricle/ventricular; LVH; left ventricular hypertrophy; NFAT; nuclear factor of activated T lymphocytes; PAAT; pulmonary artery acceleration time; PAH; pulmonary arterial hypertension; RV;
Nitric oxide-proton stimulation of trigeminal ganglion neurons increases mitogen-activated protein kinase and phosphatase expression in neurons and satellite glial cells
Keywords: MAP kinase; MAP kinase phosphatase; trigeminal ganglion neurons; nitric oxide; protons; glia; CGRP; calcitonin gene-related peptide; DAPI; 4â²,6-diamidino-2-phenylindole; ERK; extracellular signal-regulated kinases; HBS; Hepes-buffered saline; Hepes; 4-(
N-truncated amyloid-β oligomers induce learning impairment and neuronal apoptosis
Keywords: Aβ; amyloid-β peptide; AD; Alzheimer's disease; cPLA2; cytosolic calcium-dependent phospholipase A2; DAPI; 4,6-diamidino-2-phenylindole; DMEM; Dulbecco's modified eagle's medium; icv; intracerebroventricular; MAFP; methyl arachidonyl fluorophosphonate;
Methylprednisolone treatment delays remote cell death after focal brain lesion
Keywords: glucocorticoids; inferior olive; pontine nuclei; glia; inflammation; remote degeneration; CTRL; control rats; DAPI; 4,6-diamidino-2-phenylindole; HCb; hemicerebellectomy; IL-1β; interleukin-1 beta; IO; inferior olive; MPSS; methylprednisolone sodium succ
Kainate and metabolic perturbation mimicking spinal injury differentially contribute to early damage of locomotor networks in the in vitro neonatal rat spinal cord
Keywords: locomotion; spinal cord injury; excitotoxicity; burst; motoneuron; BSA; bovine serum albumin; CCF; cross-correlation function; CTRL; control; CV; coefficient of variation; DAPI; 4â²,6-diamidino-2-phenylindole; DR; dorsal root; FFT; fast Fourier transform
MicroRNAs Are Differentially Expressed in Ulcerative Colitis and Alter Expression of Macrophage Inflammatory Peptide-2α
Keywords: CD; Crohn's disease; DAPI; 4â²,6-diamidino-2-phenylindole; DMEM; Dulbecco's modified Eagle medium; IBD; inflammatory bowel disease; IBS; irritable bowel syndrome; IC; infectious colitis; MC; microscopic colitis; miRNA; microRNA; UC; ulcerative colitis; M
4′,6-Diamidino-2-phenylindole (DAPI) induces bundling of Escherichia coli FtsZ polymers inhibiting the GTPase activity
Keywords: EcFtsZ; Bundling; Polymerization; DAPI; GTPase
Chromosome observation method at metaphase and pro-metaphase stages in diploid and octoploid strawberries
Keywords: Chromosome; DAPI; Fragaria×ananassa; Fragaria vesca; Strawberry
A simple, robust and versatile method to characterise intracellular parasitism
Keywords: Ai; association index; AO; acridine orange; BSA; bovine serum albumin; DAPI; 4â²,6-diamidino-2-phenylindole; DIC; differential interference contrast; GFP; green fluorescent protein; HASPB; hydrophilic acylated surface protein B; MOI; multiplicity of infe
Cell death in response to osmotic and salt stresses in two rice (Oryza sativa L.) ecotypes
Keywords: TUNEL; DAPI; Cell death; Plant abiotic stress tolerance
Bone marrow-derived osteoblast progenitor cells in circulating blood contribute to ectopic bone formation in mice
Keywords: OPCs; osteoblast progenitor cells; BMPs; bone morphogenetic proteins; MOPCs; marrow-derived OPCs; GFP; green fluorescent protein; BMT; bone marrow transplantation; PBMNCs; peripheral blood mononuclear cells; DAPI; 6-diamidino-2-phenylindole; FITC; fluores
Expression of mKirre in the developing sensory pathways: Its close apposition to nephrin-expressing cells
Keywords: retinae; olfactory epithelia; cochleae; trigeminal ganglia; in situ hybridization; immunohistochemistry; bc; basal cells; BSA; bovine serum albumin; CN; cochlear nucleus; DAPI; 4â²,6-diamidino-2-phenylindole dihydrochloride; Duf; dumbfounded; E; embryoni
Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis
Keywords: Malassezia pachydermatis; Basidiomycetous yeast; Cytoskeleton; Microtubule; Actin; Ultrastructure; DAPI; 4â²,6-diamidino-2-phenylindole dihydrochloride; BSA; bovine serum albumin;
Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction
Keywords: ts; temperature-sensitive; Mog1; multicopy suppressor of ts gsp1; Opi3; overproducer of inositol; NTF2; nuclear transport factor; GST; glutathione S-transferase; DAPI; 4'6-diamidino-2-phenylindoleCid13; Crp79; Ntf2p/Nxt2; Ran; Ssp1
Poxvirus-derived cytokine response modifier A (CrmA) does not protect against focal cerebral ischemia in mice
Keywords: ABC; avidin-biotin-peroxidase complex (ABC); DAPI; 4â²,6-diaminido-2-phenylidole; FITC; fluorescein isothiocyanate; LDF; Laser Doppler flow; MCA; middle cerebral artery; NGS; normal goat serum; PBS; phosphate-buffered saline; PBS-T; phosphate-buffe
Repetitive DNA, molecular cytogenetics and genome organization in the King scallop (Pecten maximus)
Keywords: bp, base pairs; DAPI; 4â²,6-diamidino-2-phenylindole; EDTA, ethylenediamine tetraacetic acid; EMBL; European Molecular Biology Laboratory; Mbp, mega (million) base pairs; PCR; polymerase chain reaction; rDNA; ribosomal DNA; SDS; sodium dodecyl sulphate;
Fluorescence microscopic visualization and quantification of initial bacterial colonization on enamel in situ
Keywords: Plaque; Pellicle; DAPI; FISH; Biofilm; Enamel; In situ
Zinc stimulates the production of toxic reactive oxygen species (ROS) and inhibits glutathione reductase in astrocytes
Keywords: 3AT; 3-amino-1,2,4-triazole; DAPI; 4â²,6-diamidino-2-phenylindole dihydrochloride; DHR123; dihydrorhodamine 123; DMEM; Dulbecco's modified Eagle medium; GPx; glutathione peroxidase; GR; glutathione reductase; GSH; glutathione; GSSG; glutathione disulfide
Evaluation of the role of Disabled-2 in nerve growth factor-mediated neurite outgrowth and cellular signalling
Keywords: 5, 7-DHT; 5, 7-dihydroxytryptamine; ApoER2; apolipoprotein E receptor 2; AR; androgen receptor; CNS; central nervous system; DAG; diacylglycerol; DAPI; 4â²,6-diamidino-2-phenylindole; LF2000; lipofectamine 2000; NGF; nerve growth factor; PAK; p21-activat
Volatilisation of metals and metalloids by the microbial population of an alluvial soil
Keywords: Biomethylation; Biohydridisation; Volatile metal(loid) derivatives; 16S rDNA; Clostridium glycolicum; Fluorescence in situ hybridisation; DAPI; 4â²; 6-diamidino-2-phenylindole; PBS; phosphate buffered saline; Metal(loid) derivatives: AsH3; arsine; MMAs;
Myoblast-based cardiac repair: Xenomyoblast versus allomyoblast transplantation
Keywords: 18; 30; 34; DAPI; 4â²,6â²-Diamidino-2-phenylindole hydrochloride; DMEM; Dulbecco's modified Eagle's medium; EF; ejection fraction; FS; fractional shortening; hSkM; human skeletal myoblast; PCR; polymerase chain reaction; rSkM; rat skeletal myoblast;
Unusual compartmentalization of CTCF and other transcription factors in the course of terminal erythroid differentiation
Keywords: ICD; interchromatin domain; RBC; red blood cells; eRBC; embryonic red blood cells; CEF; chicken embryonic fibroblasts; DAPI; 4,6-diamino-2-phenylindole; CTCF; Interchromatin domain; Erythrocytes; Nuclear compartments;
Bone Morphogenetic Protein 4 Expressed in Esophagitis Induces a Columnar Phenotype in Esophageal Squamous Cells
Keywords: aRNA; antisense RNA; BE; Barrett's esophagus; BMP-4; bone morphogenetic protein 4; CK; cytokeratin; DAPI; 4â²,6-diamidino-2-phenylindole; SAGE; serial analysis of gene expression; TGF; transforming growth factor;