کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10816004 | 1058534 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of the role of Disabled-2 in nerve growth factor-mediated neurite outgrowth and cellular signalling
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کلمات کلیدی
ApoER2PLC-γRho-kinasePTBDAPIPMSFPAKNGFPKC5, 7-DHT4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولCNS - دستگاه عصبی مرکزیdiacylglycerol - دیسیل گلیسیرینNeurite outgrowth - رشد عصبیDAG - روزPC12 cells - سلول های PC12central nervous system - سیستم عصبی مرکزیnerve growth factor - فاکتور رشد عصبphospholipase C-γ - فسفولیپاز C-γPhenylmethylsulfonylfluoride - فنیل متیل سولفونیل فلوئوریدlipofectamine 2000 - لیپوفکتامین 2000Disabled-2 - معلول 2Protein kinase C - پروتئین کیناز سیp21-activated kinase - کیناز فعال p21Androgen Receptor - گیرنده آندروژنیApolipoprotein E receptor 2 - گیرنده آپولیپوپروتئین E 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of the role of Disabled-2 in nerve growth factor-mediated neurite outgrowth and cellular signalling Evaluation of the role of Disabled-2 in nerve growth factor-mediated neurite outgrowth and cellular signalling](/preview/png/10816004.png)
چکیده انگلیسی
Disabled-2 (DAB2) is an adapter protein that plays a key role in cell proliferation and differentiation. We reported here that DAB2 is expressed in various regions of rat central nervous system and is most abundant in the olfactory bulb. The up-regulation of DAB2 upon 5,7-dihydroxytryptamine-induced spinal cord lesion implicates that DAB2 may participate in the regulation of neuronal plasticity. To investigate DAB2 function in the regulation of neurite outgrowth, the rat p59 and p82 form of DAB2 was individually and stably expressed in the PC12 cells. Both p59 and p82 inhibited nerve growth factor (NGF)-induced neurite outgrowth concomitantly with a decrease in the expression of neuron-specific cytoskeleton protein β-tubulin III. To unveil the molecular mechanism of DAB2 in NGF signaling, we found that RhoA-GTPase activity was up-regulated in DAB2 stable lines whereas the Ras/MAPK and PI3-kinase/Akt signaling was not affected. The inhibitory effect of DAB2 on NGF-mediated neurite outgrowth was reversed by the pretreatment of Rho-kinase (ROCK) inhibitor Y27632, implicating that DAB2 modulates RhoA/ROCK signaling. Together, this study defines a role of DAB2 in the control of neuronal plasticity and demonstrates for the first time that DAB2 is a negative regulator in NGF-mediated neurite outgrowth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 19, Issue 6, June 2007, Pages 1339-1347
Journal: Cellular Signalling - Volume 19, Issue 6, June 2007, Pages 1339-1347
نویسندگان
Ching-Hui Huang, Ju-Chien Cheng, Jin-Chung Chen, Ching-Ping Tseng,