Development of a novel cell-based assay to monitor the transactivation activity of the HSV-1 protein ICP0
Keywords: HSV; ICP0; GFP; Viral transcription; Cell-based assay;
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Optimizing tamoxifen-inducible Cre/loxp system to reduce tamoxifen effect on bone turnover in long bones of young mice
Keywords: BV; bone volume; Cre; Cre recombinase; Col1; type I collagen; mutant ER; mutant estrogen receptor; GFP; green fluorescent protein; I.P.; intraperitoneal; microCT; micro-computed tomography; WT; wild-type; TAM; tamoxifen; Tamoxifen; Col1a1 (2.3Â kb); CreER
Medaka vasa gene has an exonic enhancer for germline expression
Keywords: EMSA; electrophoresis mobility shift assay; GFP; green fluorescent protein; PAC; puromycin acetyltransferase; PF; PAC:GFP fusion; pf; gene encoding PF; Exonic enhancer; Gene transcription; Germline; Vasa; V35;
Uses and limitations of green fluorescent protein as a viability marker in Enterococcus faecalis: An observational investigation
Keywords: GFP; Fluorescence; Viability; Enterococcus faecalis; Real-time imaging; Oxidizing agents;
Immunogenicity and safety of an E. coli-produced bivalent human papillomavirus (type 16 and 18) vaccine: A randomized controlled phase 2 clinical trial
Keywords: Human papillomavirus; Vaccine; Immunogenicity; Safety; Randomized controlled clinical trial; CFDA; Chinese Food and Drug Administration; CIs; confidential intervals; eVLP-based ELISA; E. coli-expressed HPV L1 VLP-based ELISA; GFP; green fluorescent protei
A new low-Ca2+ affinity GAP indicator to monitor high Ca2+ in organelles by luminescence
Keywords: GAP; GFP-Aequorin Protein; GA; GFP-aequorin; ER; endoplasmic reticulum; Go; Golgi apparatus; tGo; trans Go; [Ca2+]ER and [Ca2+]Go; Ca2+ concentrations in ER or Go; SERCA; sarco-endoplasmic reticulum Ca2+ ATPase; TBH; 2,5-di(tert-butyl)-1,4-benzohydroquino
Essential function of Aco2, a fusion protein of aconitase and mitochondrial ribosomal protein bL21, in mitochondrial translation in fission yeast
Keywords: EMM; Edinburgh minimal medium; MTS; mitochondrial targeting sequence; NLS; nuclear localization signal; GFP; green fluorescence protein; EGFP; enhanced GFP; Gene fusion; Sub-cellular localization; Dual localized protein; Dual function protein; Mitochondri
Cloning and characterization of the Escherichia coli Heptosyltransferase III: Exploring substrate specificity in lipopolysaccharide core biosynthesis
Keywords: LPS; lipopolysaccharide; GTs; glycosyltransferases; Hep; l-glycero-d-manno-heptose; HepI; Heptosyltransferase I; HepIII; Heptosyltransferase III; ADP-Hep; adenosine diphosphate-l-glycero-d-manno-heptose; Kdo; 3-deoxy-d-manno-oct-2-ulosonic acid; GFP; gree
The role of N-glycan in folding, trafficking and pathogenicity of myelin oligodendrocyte glycoprotein (MOG)
Keywords: CNS; central nervous system; EAE; experimental autoimmune encephalomyelitis; ER; endoplasmic reticulum; GAPDH; glyceraldehyde phosphate dehydrogenase; GFP; green fluorescent protein; MOG; myelin oligodendrocyte glycoprotein. MOG35-55 sequence: MetGluVal
The unfulfilled gene and nervous system development in Drosophila
Keywords: Burs; bursicon; CCAP; crustacean cardioactive peptide; DBD; DNA-binding domain; EH; eclosion hormone; fax-1; fasciculation of axons defective-1; GFP; green fluorescent protein; KC; Kenyon cells; LBD; ligand-binding domain; MARCM; mosaic analysis with a re
Imp2, the PSTPIP homolog in fission yeast, affects sensitivity to the immunosuppressant FK506 and membrane trafficking in fission yeast
Keywords: Proline-serine-threonine phosphatase interacting protein; Fission yeast; Cytokinesis; Membrane trafficking; Calcineurin; PSTPIP1; proline-serine-threonine phosphatase interacting protein 1; YPD; yeast extract-peptone-dextrose; EMM; Edinburgh minimal mediu
Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP
Keywords: Angiogenesis; Mechanotransduction; Sprouting; Furin; ECs; endothelial cells; MT1-MMP; membrane type-1 matrix metalloproteinase; ECM; extracellular matrix; WSS; wall shear stress; S1P; sphingosine 1-phosphate; PC; proprotein convertase; 3D; three-dimension
A gene locus for targeted ectopic gene integration in Zymoseptoria tritici
Keywords: sdi1; succinate dehydrogenase 1; sdhb; succinate dehydrogenase subunit B; Ip; iron-sulfur protein; sdi1R; carboxin-resistant; GFP; green fluorescent protein; RFP; red fluorescent protein; eGFP; enhanced green fluorescent protein; AcGFP; Aequorea coerule
Extinction and reinstatement to cocaine-associated cues in male and female juvenile rats and the role of D1 dopamine receptor
Keywords: Adolescence; Cocaine; D1; Extinction; Reinstatement; CK; CamKIIα; CS; conditioned stimulus; GFP; green fluorescent protein; ilPFC; infralimbic prefrontal cortex; mPFC; medial prefrontal cortex; plPFC; prelimbic prefrontal cortex; US; unconditioned stimul
VvGONST-A and VvGONST-B are Golgi-localised GDP-sugar transporters in grapevine (Vitis vinifera L.)
Keywords: CSL; cellulose synthase-like; ER; endoplasmic reticulum; GDP; guanosine diphosphate; GFP; green fluorescent protein; GONST; Golgi Nucleotide Sugar Transporter; GTs; glycosyltransferases; NDP; nucleotide diphosphate; NSTs; nucleotide-sugar transporters; UD
CCAAT/enhancer-binding protein CEBP-2 controls fat consumption and fatty acid desaturation in Caenorhabditis elegans
Keywords: Caenorhabditis elegans (C. elegans); CEBP-2; Beta-oxidation; Lipid synthesis; C/EBPs; CCAAT/enhancer-binding proteins; CGC; Caenorhabditis Genetics Center; GFP; green fluorescent protein; NGM; nematode growth medium; ORO; Oil-Red-O; QRT-PCR; quantitative
Infection of the upper respiratory tract of hamsters by the bovine parainfluenza virus type 3 BN-1 strain expressing enhanced green fluorescent protein
Keywords: Parainfluenza virus; GFP; Pathogenesis; Reverse genetics; Hamster; Respiratory tract; Ciliated epithelial cell; Bovine respiratory disease complex;
The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1â³-pase catalytic-site, critical for viral RNA replication
Keywords: AA; Amino acid; Appr-1â³-pase; ADP-ribose-1â³-monophosphatase; Asn; Asparagine; CoV; Coronavirus; FACS; Fluorescence activated cell sorting; GFP; Green fluorescent protein; Gly; Glycine; HEV; Hepatitis E virus; His; Histidine; ORF; Open reading frame; P
Depletion of Foxp3+ regulatory T cells increases severity of mechanical allodynia and significantly alters systemic cytokine levels following peripheral nerve injury
Keywords: Treg; DEREG; Chronic constriction injury; Neuropathic pain; Systemic cytokines; CCI; Chronic constriction injury; PSNL; partial sciatic nerve ligation; DEREG; DEpletion of REGulatory T cells; DT; diphtheria toxin; GFP; green fluorescent protein; SN; sciat
A treadmill exercise reactivates the signaling of the mammalian target of rapamycin (mTor) in the skeletal muscles of starved mice
Keywords: mTor; mammalian target of rapamycin; GFP; green fluorescent protein; LC3; microtubule-associated protein IA/IB light chain 3; IGF-1; insulin-like growth factor 1; Autophagy; Exercise; Treadmill; Skeletal muscle; mTor; GFP-LC3;
Autophagic flux determination in vivo and ex vivo
Keywords: ATP; adenosine triphosphate; BBB; blood brain barrier; BRB; blood retinal barrier; BGT; BSA/glycine/Triton X-100; BSA; bovine serum albumin; CNS; central nervous system; DABCO; 1,4-diazabicyclo[2.2.2]octane; DAPI; 4,6-diamidino-2-phenylindole dihydrochlor
Function, pharmacological correction and maturation of new Indian CFTR gene mutations
Keywords: CBAVD; congenital bilateral absence of the vas deferens; CF; cystic fibrosis; CFTR; cystic fibrosis transmembrane conductance regulator; GFP; green fluorescent protein; WT-CFTR; wild-type CFTR; Missense CF mutations; India; L69H-CFTR; S549N-CFTR; Low temp
Yeast recombination-based cloning as an efficient way of constructing vectors for Zymoseptoria tritici
Keywords: YRBC; yeast recombination-based cloning; sdi1; succinate dehydrogenase; hph; hygromycin phosphotransferase; nptII; neomycin phosphotransferase; bar; bialaphos resistant gene; GFP; green-fluorescent protein; Zt; Zymoseptoria tritici; RB and LB; right and l
Therapeutic ultrasound: Increased HDL-Cholesterol following infusions of acoustic microspheres and apolipoprotein A-I plasmids
Keywords: ApoA-I; Optisonâ¢; Acoustic microspheres; Ultrasound; Drug delivery; HDL-C; Hypo-alphalipoproteinemia; ApoA-I; apolipoprotein A-I; HDL-C; High density lipoprotein cholesterol; LDL-C; Low density lipoprotein cholesterol; MI; Mechanical index; GFP; Green f
A single mutation in helix 8 enhances the angiotensin II type 1a receptor transport and signaling
Keywords: GPCR; G protein-coupled receptor; Ang II; angiotensin II; AT1R; angiontensin II type 1 receptor; AT1aR; angiontensin II type 1a receptor; AT2R; angiontensin II type 2 receptor; MAPK; mitogen-activated protein kinase; ERK1/2; extracellular signal-regulated
Mitophagy and mitochondrial dynamics in Saccharomyces cerevisiae
Keywords: AIM; Atg8 family interacting motif; ALP; alkaline phosphatase; Ape1; aminopeptidase I; ATG; autophagy-related; CCCP; carbonyl cyanide m-chlorophenylhydrazone; CJ; crista junction; CM; cristae membrane; CMA; chaperone-mediated autophagy; Cvt; cytoplasm to
Research ArticleS100A4 promotes liver fibrosis via activation of hepatic stellate cells
Keywords: HSCs; hepatic stellate cells; ECM; extracellular matrix; HA; hyaluronic acid; α-SMA; alpha-smooth muscle actin; TIMP; tissue inhibitors of matrix metalloproteinase; MMP; metalloproteinase; CCl4; carbon tetrachloride; GFP; green fluorescent protein; NPCs;
Overexpressed Arabidopsis Annexin4 accumulates in inclusion body-like structures
Keywords: Annexins; Annexin4; Inclusion body; Aggresome; Arabidopsis thaliana; AnnAt4; Arabidopsis Annexin 4; CTP; cytidine triphosphate; DIC; differential interference contrast; GFP; green fluorescent protein;
TDP-35 sequesters TDP-43 into cytoplasmic inclusions through binding with RNA
Keywords: ALS; amyotrophic lateral sclerosis; DNase; deoxyribonuclease; FISH; fluorescent in situ hybridization; FTLD; frontotemporal lobar degeneration; GFP; green fluorescent protein; IF; immunofluorescence; ITC; isothermal titration calorimetry; RIP; RNA immunop
Autophagy in zebrafish
Keywords: 3MA; 3-methyladenin; AC; adenylyl cyclase; AMPK; adenosine monophosphate-dependent kinase; Atg; autophagy related gene; BafA1; Bafilomycin A1; CMV; cytomegalovirus; CQ; chloroquine; dpa; days post amputation; dpf; days post fertilization; ERK; extracellul
Subcellular localization and internalization of the vasopressin V1B receptor
Keywords: AVP; arginine-vasopressin; C-terminus; carboxyl-terminus; CHO; Chinese hamster ovary; DAB; 3,3â²-diaminobenzidine; DMEM; Dulbecco's modified Eagle's medium; GFP; green fluorescent protein; GPCRs; G-protein coupled receptors; HEK; human embryonic kidn
Modulation of TCR responsiveness by the Grb2-family adaptor, Gads
Keywords: DAG; diacylglycerolDSB, double strand break; Gads; Grb2-related adaptor downstream of Shc; Indel; insertion and deletion mutation; GFP; green fluorescent protein; Grap; Grb2-related adaptor protein; Grb2; growth factor receptor-bound 2; IRES; internal rib
MAPK-dependent phosphorylation modulates the activity of Suppressor of Hairless in Drosophila
Keywords: ANK; ankyrin repeats domain of Notch; Bx; Beadex; CSL; acronym of CBF1 (human C-promoter binding factor 1) Su(H) (Suppressor of Hairless, Drosophila melanogaster) and Lag-1 (lin-12 and Glp-1 phenotype, Caenorhabditis elegans); EGFR; epidermal growth facto
BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion
Keywords: AIF; apoptosis inducible factor; AP-1; activator protein-1; AKT; protein kinase B; Apaf-1; apoptosis protease activating factor-1; BAD; Bcl-2-associated death promoter; BCL-2; B-cell lymphoma 2; BCLxL; B-cell lymphoma-extra large; BH3; Bcl-2 homology doma
Dying tumor cells stimulate proliferation of living tumor cells via caspase-dependent protein kinase Cδ activation in pancreatic ductal adenocarcinoma
Keywords: Pancreatic cancer; Apoptosis; Repopulation; Caspase; PKCδ; Akt/MAPK; PKC; protein kinase C; MAPK; mitogen-activated protein kinase; JNK1/2; c-Jun N-terminal kinase/stress-activated protein kinase; iPLA2; calcium-independent phospholipase A2; PGE2; prosta
Ceramide signalling impinges on Sit4p and Hog1p to promote mitochondrial fission and mitophagy in Isc1p-deficient cells
Keywords: ALP; alkaline phosphatase; ATP; adenosine triphosphate; CLS; chronological lifespan; COX; cytochrome c oxidase; CR; caloric restriction; EDTA; ethylenediamine tetraacetic acid; ER; endoplasmic reticulum; FCCP; carbonyl cyanide p-(trifluoromethoxy)phenylhy
Paracrine co-delivery of TGF-β and IL-2 using CD4-targeted nanoparticles for induction and maintenance of regulatory T cells
Keywords: Autoimmunity; Drug delivery; Immunomodulation; Nanoparticles; TGF-β; Interleukin-2; CD; cluster of differentiation; DMSO; dimethylsulfoxide; ELISA; enzyme-linked immunosorbent assay; FACS; fluorescent activated cell sorting; FBS; fetal bovine serum; GFP;
Thioester-containing proteins of the tick Ixodes ricinus: Gene expression, response to microbial challenge and their role in phagocytosis of the yeast Candida albicans
Keywords: GFP; green fluorescent protein; IrA2M1, 2, 3; Ixodes ricinus α2-macroglobulin 1, 2, 3, respectively; IrC3-1, -2, -3; I.âricinus C3- complement component 1, 2, 3, respectively; IrMcr1, 2; I.âricinus macroglobulin complement related 1,2, respectively;
Research PaperDepressive-like phenotype induced by AAV-mediated overexpression of human α-synuclein in midbrain dopaminergic neurons
Keywords: ACTH; adrenocorticotropic hormone; AAV; adeno-associated virus; Arc; activity-regulated cytoskeleton-associated protein; BDNF; brain-derived neurotrophic factor; GFP; green fluorescent protein; HPA; hypothalamic-pituitary-adrenal; KPBS; potassium-phosphat
The YhhN protein of Legionella pneumophila is a Lysoplasmalogenase
Keywords: AA; arachidonic acid; ADH; alcohol dehydrogenase; BSA; bovine serum albumin; DHA; docosahexaenoic acid; DTT; dithiothreitol; GFP; Green Fluorescent Protein; HDL; high-density lipoproteins; His; histidine; NADH; nicotinamide adenine dinucleotide; KPi; pota
Structure-activity studies of Wnt/β-catenin inhibition in the Niclosamide chemotype: Identification of derivatives with improved drug exposure
Keywords: APC; adenomatous polyposis coli; CRC; colorectal cancer; Dvl; Dishevelled; LEF/TCF; lymphoid enhancer factor/T cell factor; Fzd1; Frizzled1; GFP; green fluorescent protein; NAFLD; non-alcoholic fatty liver disease; NASH; non-alcoholic steatohepatitis; SAR
Calcineurin B homologous protein 3 negatively regulates cardiomyocyte hypertrophy via inhibition of glycogen synthase kinase 3 phosphorylation
Keywords: Akt; protein kinase B; ANP; atrial natriuretic peptide; CaMKII; Ca2Â +/calmodulin-dependent protein kinase II; CHP3; calcineurin B homologous protein 3; DAPI; 4â²,6-diamidino-2-phenylindole dihydrochloride; DMEM; Dulbecco's modified Eagle's medium; FBS;
GDNF-secreting mesenchymal stem cells provide localized neuroprotection in an inflammation-driven rat model of Parkinson's disease
Keywords: ANOVA; analysis of variance; ELISA; enzyme-linked immunosorbent assay; GDNF; glial cell line-derived neurotrophic factor; GFP; green fluorescent protein; HEPES; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; LPS; lipopolysaccharide; MSCs; mesenchymal
Critical role of the neural pathway from the intermediate medial mesopallium to the intermediate hyperpallium apicale in filial imprinting of domestic chicks (Gallus gallus domesticus)
Keywords: A3V; avian adeno-associated virus; AMHA; anterior medial hyperpallium apicale; ANOVA; analysis of variance; CTB; cholera toxin subunit B; DLA; nucleus dorsolateralis anterior thalami; EGFP; enhanced green fluorescence protein; GFP; green fluorescence prot
The tomato DDI2, a PCNA ortholog, associating with DDB1-CUL4 complex is required for UV-damaged DNA repair and plant tolerance to UV stress
Keywords: Tomato (Solanum lycopersicum); CRL4 ubiquitin E3 ligase; DDI2; PCNA; UV stress; DNA damage repair; CaMV; cauliflower mosaic virus; GFP; green fluorescent protein; NER; nucleotide excision repair; OE; overexpression; ORF; open reading frame; UV; ultraviole
The yeast Saccharomyces cerevisiae: An overview of methods to study autophagy progression
Keywords: 3-MA; 3-methyladenine; Atg; autophagy related; CRISPR; Clustered Regularly Interspaced Short Palindromic Repeats; GFP; green fluorescent protein; PAS; phagophore assembly site; PKA; protein kinase A; PMN; piecemeal microautophagy of the nucleus; TEM; tran
DNA replication restart and cellular dynamics of Hef helicase/nuclease protein in Haloferax volcanii
Keywords: Archaea; DNA replication restart; GFP; Hef;
α-Synuclein-induced internalization of NMDA receptors in hippocampal neurons is associated with reduced inward current and Ca2+ influx upon NMDA stimulation
Keywords: AD; Alzheimer's disease; AM; acetoxymethyl; AS; antisense sequence; BCA; bicinchoninic acid; DLB; dementia with Lewy bodies; EDTA; ethylenediaminetetraacetic acid; EGTA; ethylenebistetraacetic acid; GFP; green fluorescent protein; HBSS; Hank's balance
Engineering batch and pulse refolding with transition of aggregation kinetics: An investigation using green fluorescent protein (GFP)
Keywords: Inclusion bodies; Renaturation; Folding; Reaction order; GFP;
Expression of vesicular glutamate transporters in transient receptor potential melastatin 8 (TRPM8)-positive dental afferents in the mouse
Keywords: CGRP; Calcitonin Gene-Related Peptide; CFA; Complete Freund's Adjuvant; Cy; Cyanine; DRG; dorsal root ganglion; EDTA; ethylenediaminetetraacetic acid; FITC; fluorescein isothiocyanate; GFP; green fluorescent protein; LM; light microscopy; NDS; normal do