دانلود مقالات ISI درباره Guhcl + ترجمه فارسی

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Engineering a Cysteine-Free Form of Human Fibroblast Growth Factor-1 for “Second Generation” Therapeutic Application

Keywords: FGF-1; disulfide; protein stability; empirical phase diagram; X-ray crystallography; cysteine-free mutant; protein engineering; cystine; FGF-1; fibroblast growth factor-1; WT; wild-type; DTNB; 5,5'-dithiobis(2-nitrobenzoic acid); GuHCl; guanidine hydrochl

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2016 سفارش ترجمه

تجدید ساختار محاسباتی تیرودوکسین بسیار حساس به تغییرات سازگاری جزئی در قالب ستون فقرات

Computational Redesign of Thioredoxin Is Hypersensitive toward Minor Conformational Changes in the Backbone Template

Keywords: REU; Rosetta energy units; PDB; Protein Data Bank; SEC; size-exclusion chromatography; GuHCl; guanidine hydrochloride; DSS; 4,4-dimethyl-4-silapentane-1-sulfonic acid; pl; isoelectric point; computational protein design; de novo protein design; Rosetta; p

دانلود رایگان متن کامل مقاله ISI 17 صفحه سال انتشار : 2016 سفارش ترجمه

Domain swapping dissection in Thermotoga maritima arginine binding protein: How structural flexibility may compensate destabilization

Keywords: TmArgBP; arginine binding protein isolated from Thermotoga maritima; TmArgBP20-233; truncated form of TmArgBP; arg-TmArgBP20-233; arginine-bound form of TmArgBP20-233; lf-TmArgBP20-233; ligand-free form of TmArgBP20-233; TmArgBP_C-ter232-246; peptide corr

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2018 سفارش ترجمه

Residual structures in the unfolded state of starch-binding domain of glucoamylase revealed by near-UV circular dichroism and protein engineering techniques

Keywords: GuHCl; guanidinium hydrochloride; SBD; starch binding domain of glucoamylase from Aspergillus niger; WT; wild type; SP; Similarity Parameter; NATA; N-acetyl-l-tryptophanamide; β-CD; β-cyclodextrin; Fluorescence; Circular dichroism; Residual structure; P

دانلود رایگان متن کامل مقاله ISI 35 صفحه سال انتشار : 2016 سفارش ترجمه

FRET analysis of CP12 structural interplay by GAPDH and PRK

Keywords: Intrinsic disorder proteins; CP12; GAPDH; PRK; Single molecule FRET; Conformational modulation; IDP; intrinsically disordered protein; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; PRK; phosphoribulokinase; GuHCl; guanidinium hydrochloride; E; energy t

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2015 سفارش ترجمه

Functional importance of a peripheral pocket in mammalian cytochrome P450 2B enzymes

Keywords: Cytochrome P450; Structure-function relationship; Site-directed mutagenesis; Monooxygenase coupling; CYP; cytochrome P450; ITC; isothermal titration calorimetry; H/D; hydrogen deuterium; DXMS; H/D exchange coupled to mass spectrometry; SNP; single nucle

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2015 سفارش ترجمه

Stability of early-stage amyloid-β(1-42) aggregation species

Keywords: AD; Alzheimer's disease; Aβ; amyloid-β protein; GuHCl; guanidine hydrochloride; HFIP; hexafluoroisopropanol; SDS; sodium dodecyl sulfate; SEC; size exclusion chromatography; ThT; thioflavin T; Amyloid-beta protein; Aggregation; Fibrillar oligomer;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Development and characterization of a stable eGFP enterovirus 71 for antiviral screening

Keywords: EV71; enterovirus 71; HTS; high-throughput screen; hpi; hour post-infection; pt; post-transfection; pi; post-infection; eGFP; enhanced green fluorescent protein; GuHCl; guanidine hydrochloride; Enterovirus; Reporter virus; High-throughput assay; Antiviral

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Structural landscape of the proline-rich domain of Sos1 nucleotide exchange factor

Keywords: Abi1; Abl interactor 1; CD; Circular dichroism; Cdc25; Cell cycle division 25; DH; Dbl homology; DLS; Dynamic light scattering; EGFR; Epidermal growth factor receptor; Eps8; EGFR kinase substrate 8; Grb2; Growth factor receptor binder 2; GuHCl; Guanidine

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Integral role of the I′-helix in the function of the “inactive” C-terminal domain of catalase-peroxidase (KatG)

Keywords: KatG; catalase-peroxidase; wtKatG; wild-type KatG; KatGN; separately expressed and isolated KatG N-terminal domain; KatGC; separately expressed and isolated KatG C-terminal domain; LL1; Large Loop 1; LL2; Large Loop 2; APx; ascorbate peroxidase; CcP; cy

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Crystallographic and spectroscopic characterizations of Sulfolobus solfataricus TrxA1 provide insights into the determinants of thioredoxin fold stability

Keywords: RMSD; root mean square deviation; PDB; Protein Data Bank; Trx; thioredoxin; TrxR; thioredoxin reductase; SsTrxA2; Sulfolobus solfataricus Trx A2; SsTrxA1; Sulfolobus solfataricus Trx A1; EcTrx; Escherichia coli Trx; TtTrx; Thermus thermophilus Trx; SpTrx;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

A fluorescent-based HPLC assay for quantification of cysteine and cysteamine adducts in Escherichia coli-derived proteins

Keywords: RP HPLC; reversed-phase high performance liquid chromatography; LC/MS; liquid chromatography/mass spectrometry; TCEP; Tris (2-carboxyethyl) phosphine; DTT; dithiothreitol; GuHCl; guanidine hydrochloride; TFA; trifluoroacetic acid; AP; acid precipitation;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

The role of a disulfide bridge in the stability and folding kinetics of Arabidopsis thaliana cytochrome c6A

Keywords: Cyt; cytochrome; At; Arabidopsis thaliana; Pl; Phormidium laminosum; Em; heme mid-point redox potential; LIP; loop insertion peptide; wt; wild-type; Amp; ampicillin; Cam; chloramphenicol; LB; lysogeny-broth; IPTG; isopropyl β-D-1-thiogalactopyranoside; G

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2012 سفارش ترجمه

Neriifolin S, a dimeric serine protease from Euphorbia neriifolia Linn.: Purification and biochemical characterisation

Keywords: BSA; bovine serum albumin; βme; β-mercaptoethanol; CBB; Coomassie brilliant blue; DFP; diisopropylfluorophosphate; DMSO; dimethylsulphoxide; DTNB; 5,5′-dithiobis-(2-nitrobenzoic acid); DTT; dithiothreitol; EDTA; ethylene di amine tetra acetic acid; EG

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

Religiosin B, a milk-clotting serine protease from Ficus religiosa

Keywords: Ficus religiosa; Latex; Milk-clotting enzyme; Religiosin B; Serine protease; BSA; bovine serum albumin; CBB; coomassie brilliant blue; DEAE; diethylaminoethyl; DMSO; dimethyl sulfoxide; DTNB; 5,5-dithiobis (2-nitrobenzoicacid); DTT; di-thiothreitol; EDTA;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

Altered cofactor binding affects stability and activity of human UDP-galactose 4′-epimerase: Implications for type III galactosemia

Keywords: ANS; 1-anilinonaphthalene-8-sulphonic acid; BS3; suberic acid bis(3-sulfo-N-hydroxysuccinimide ester); Gal-1P; galactose 1-phosphate; Glc-1P; glucose 1-phopshate; GALE; UDP-galactose 4′-epimerase; GuHCl; guanidine hydrochloride; hGALE; human GALE; FRET;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2012 سفارش ترجمه

Small-Angle X-ray Scattering and Single-Molecule FRET Spectroscopy Produce Highly Divergent Views of the Low-Denaturant Unfolded State

Keywords: smFRET; single-molecule Förster resonance energy transfer; SAXS; small-angle X-ray scattering; GuHCl; guanidine hydrochloride; APS; Advanced Photon Source; CHESS; Cornell High Energy Synchrotron Source; physiological unfolded state; hydrophobic collapse;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2012 سفارش ترجمه

Expression, purification and preliminary biochemical and structural characterization of the leucine rich repeat namesake domain of leucine rich repeat kinase 2

Keywords: BS3; bis(sulfosuccinimidyl) suberate; CD; circular dichroism; Chaps; 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate; CL; cross-linking; DDM; n-Dodecyl-β-d-maltopyranoside; DLS; dynamic light scattering; DTT; dithiothreitol; EDC; 1-ethyl-3-(3-

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2012 سفارش ترجمه

Comparison of activity and conformational changes of ficin during denaturation by urea and guanidine hydrochloride

Keywords: ANS; 8-anilino-1-naphthalene-sulfonic acid; CD; circular dichrosism; GuHCl; guanidine hydrochloride; Fu; fraction unfolded; Ksv; Stern-Volmer constant; Cysteine protease; Ficin; Guanidine hydrochloride; Protein denaturation; Stability; Urea; Unfolding;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2011 سفارش ترجمه

Crystal structure of the human collagen XV trimerization domain: A potent trimerizing unit common to multiplexin collagens

Keywords: COL; collagenous; NC; non-collagenous; NC1; carboxyl-terminal non-collagenous domain; GuHCl; Guanidine Hydrochloride; Collagen XV; Collagen XVIII; Trimerization domain; Non-collagenous domain; Multiplexin;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2011 سفارش ترجمه

Effects of glycosylation on the stability and flexibility of a metastable protein: The human serpin α1-antitrypsin

Keywords: α1-AT; α1-antitrypsin; HPα1-AT; human plasma α1-antitrypsin; RCα1-AT; recombinant α1-antitrypsin; RCL; reactive center loop; GuHCl; guanidine hydrochloride; HXMS; hydrogen/deuterium exchange mass spectrometry; Serpin; Antitrypsin; Glycosylation; Hyd

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2011 سفارش ترجمه

Biochemical characterization of a peroxidase isolated from Caribbean plant: Euphorbia cotinifolia

Keywords: BSA; bovine serum albumin; DMSO; dimethyl sulfoxide; GuHCl; guanidine hydrochloride; H2O2; hydrogen peroxide; PAGE; polyacrylamide gel electrophoresis; SDS; sodium dodecyl sulfate; TEMED; tetramethylethylenediamine; EDTA; ethylenediaminetetraacetic acid;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2011 سفارش ترجمه

Characterization of functional intermediates of endoglucanase from Aspergillus aculeatus during urea and guanidine hydrochloride unfolding

Keywords: CMC; carboxymethyl cellulose; GuHCl; guanidine hydrochloride; ANS; 1-anilino-8-naphthalene sulfonic acid; DNS; 2,4 dinitro salicylic acid; Tm; apparent thermal transition temperature; CD; circular dichroism; Endoglucanase; Urea; GuHCl; Conformation;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2010 سفارش ترجمه

Dubiumin, a chymotrypsin-like serine protease from the seeds of Solanum dubium Fresen

Keywords: Solanum dubium Fresen; Solanaceae; Purification; Characterization; Serine protease; Dubiumin; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; PMSF; phenylmethanesulfonyl fluoride; GuHCl; guanidine hydrochloride; EDTA; ethylenediam

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2009 سفارش ترجمه

Conformational stability of α-amylase from malted sorghum (Sorghum bicolor): Reversible unfolding by denaturants

Keywords: Sorghum α-amylase; Conformational stability; Cysteine oxidation; Protein unfolding; Electrostatic interactions; TEMED; N,N,N′,N′-tetramethyl ethylenediamine; Hepes; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; GuHCl; guanidine hydrochloride; D

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2009 سفارش ترجمه

Enhancement of nuclease P1 activity in low concentration of denaturants

Keywords: Nuclease P1; Urea; GuHCl; Enhanced enzyme activity; Molten globule

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2008 سفارش ترجمه

Helix-rich transient and equilibrium intermediates of equine β-lactoglobulin in alkaline buffer

Keywords: ELG; equine β-lactoglobulin; CD; circular dichroism; far-UV; far-ultraviolet; EGOH; ethylene glycol; BLG; bovine β-lactoglobulin; GuHCl; guanidine hydrochloride.; Protein folding; Equine β-lactoglobulin; Stopped-flow; Equilibrium intermediate; Transien

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2008 سفارش ترجمه

Indicain, a dimeric serine protease from Morus indica cv. K2

Keywords: BAPA; NR-benzoylarginine p-nitroanilide; BLAST; basic local alignment search tool; BSA; bovine serum albumin; DFP; diisopropylfluorophosphate; DMSO; dimethyl sulfoxide; DTNB; 5,5μ-dithiobis(2-nitrobenzoic acid); E-64; 3S)-3-(N-{(S)-1-[N-(4-guanidinobutyl

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2008 سفارش ترجمه

Role of the N-terminal region for the conformational stability of esterase 2 from Alicyclobacillus acidocaldarius

Keywords: EST2; esterase from Alicyclobacillus acidocaldarius; HSL; hormone sensitive lipase family; GuHCl; guanidine hydrochloride; pNP; p-nitrophenyl; CD; circular dichroism; DSC; differential scanning calorimetry; HPLC; high performance liquid chromatography; SD

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii

Keywords: Euphorbia milii; Euphorbiaceae; Serine proteases; Plant endopeptidases; Milin; Anti-milin; NEM; N-ethyl maleimide; EDTA; ethylene diammine tetraacetic acid; EGTA; ethylene glycol-bis (α-amino ethyl ether); BAPA; NR-benzoylarginine p-nitroanilide; DFP; di

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2006 سفارش ترجمه

The structure of human apolipoprotein E2, E3 and E4 in solution. 2. Multidomain organization correlates with the stability of apoE structure

Keywords: apoE; apolipoprotein E; apo; apolipoprotein; CD; circular dichroism; CT; carboxyl-terminal; DTT; dithiothreitol; GuHCl; guanidine hydrochloride; N; native state of the protein; NT; amino-terminal; p; plasma; r; recombinant; Rs; Stokes radius; U; unfolded

دانلود رایگان متن کامل مقاله ISI 16 صفحه سال انتشار : 2006 سفارش ترجمه

The first archaeal agmatinase from anaerobic hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization

Keywords: DMSO; dimethyl sulfoxide; GuHCl; guanidine hydrochloride; Archaea; Agmatinase; Pyrococcus horikoshii; Polyamine; Agmatine;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2005 سفارش ترجمه

The equilibrium unfolding of MerP characterized by multivariate analysis of 2D NMR data

Keywords: Multivariate NMR data analysis; Protein folding; PCA; PLS; GuHCl;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2005 سفارش ترجمه

Two distinct intermediates of trigger factor are populated during guanidine denaturation

Keywords: Chaperone; Folding intermediate; Pre-molten globule; Prolyl isomerase; Protein folding; Trigger factor; ANS; 1-anilininonaphthalene-8-sulfonate; DSS; disuccinimidyl suberate; GuHCl; guanidine hydrochloride; MG; molten globule; PPIase; peptidyl-prolyl ci

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2005 سفارش ترجمه

FMN binding and unfolding of Desulfovibrio desulfuricans flavodoxin: “hidden” intermediates at low denaturant concentrations

Keywords: CD; circular dichroism; FMN; flavin mononucleotide; ITC; isothermal titration calorimetry; KD; dissociation constant; GuHCl; guanidine hydrochloride; ANS; 8-anilinonaphthalene-1sulfonic acid; Flavin mononucleotide; Flavodoxin; Protein folding; Isothermal

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2005 سفارش ترجمه