کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10162033 | 1114312 | 2015 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel Layer-by-Layer Deposition Technique for the Preparation of Double-Chambered Nanoparticle Formulations
ترجمه فارسی عنوان
تکنیک رسوب گذاری لایه های لایه ای برای تهیه فرمولاسیون نانو ذرات دو بعدی
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
چکیده انگلیسی
In this work, we report a novel method of layer-by-layer (LbL) deposition using concentration tubes that enables faster process and less damage to fragile nanocores than previously described methods. Such methods are generally based on continuous cycles of centrifugation/resuspension for long times and at high speeds, which may eventually lead to the aggregation of the deflocculated suspension of nanoparticles into a compact, non-resuspendable cake. The new method was applied to the preparation of a double-chambered nanocarrier system, which was successfully loaded with a fluorescently labeled model protein (lysozyme) and a model small molecule (fluorescein) in two defined and separate compartments, namely the poly lactide-co-glycolide (PLGA) core (â¼110Â nm) and an outer shell obtained by LbL surface coating. The new method yielded stable suspensions of drug-loaded, LbL-coated PLGA nanoparticles, while centrifugation at high speeds and long time intervals leads to a compact cake of non-resuspendable aggregates. These nanocarriers were taken up by MDCK cells in vitro, where a colocalization of both model compounds was shown by confocal imaging.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 104, Issue 8, August 2015, Pages 2637-2640
Journal: Journal of Pharmaceutical Sciences - Volume 104, Issue 8, August 2015, Pages 2637-2640
نویسندگان
Omar S. Sakr, Olivier Jordan, Gerrit Borchard,