کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10162678 1114349 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mechanism of Protein Stabilization by Trehalose During Freeze-Drying Analyzed by In Situ Micro-Raman Spectroscopy
ترجمه فارسی عنوان
مکانیسم تثبیت پروتئین توسط ترهالوز در طی خشک کردن خشک با استفاده از اسپکتروسکوپ میکرو رامان در حالت
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
چکیده انگلیسی
Raman investigations were performed in situ during freeze-drying of two model proteins, lysozyme and chymotrypsinogen. The structures of proteins dissolved in 0-30 wt % solutions of trehalose in D2O were monitored with the fingerprint (800-1800 cm− 1) spectrum, simultaneously with freezing, ice sublimation, and water desorption analyzed in the O-D stretching (2200-2700 cm− 1) region. In the absence of trehalose, the main changes were detected at the end of primary drying, and correspond to distortion and disordering of secondary structures. A stabilizing effect of trehalose was evidenced in primary and secondary drying stages. Raman images were calculated after freezing and primary drying, providing the distributions of trehalose, water, and protein which occur during the first two stages of the lyophilization cycle. Raman images show a slight heterogeneity in the degree of protein denaturation at the end of primary drying, in relation with the structure of the frozen product observed during freezing. The ability of trehalose to make the protein more rigid was determined as responsible for the protein stabilization during a lyophilization cycle. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:2484-2494, 2013
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 102, Issue 8, August 2013, Pages 2484-2494
نویسندگان
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