کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10211243 | 1646120 | 2018 | 51 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Excitation and emission wavelength dependence of fluorescence spectra in whole cells of the cyanobacterium Synechocystis sp. PPC6803: Influence on the estimation of Photosystem II maximal quantum efficiency
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کلمات کلیدی
PBUPhycobilisomePS IINPQDASPhycocyaninChlAPCFWHMPBSPS I - PS منallophycocyanin - آلوفوکسیانینLHC - برخورد دهنده بزرگ هادرون fluorescence detection - تشخیص فلورسنتPhotochemical efficiency - راندمان عکس شیمیاییChlorophyll - سبزینه یا کلروفیلCyanobacteria - سیانوباکتریهاDecay associated spectra - طیف مربوطه را کاهش دهیدfull width at half maximum - عرض کامل در نیمی از حداکثرQuantum yield - عملکرد کوانتومیFluorescence quantum yield - عملکرد کوانتومی فلورسانسphotosystem I - عکس سیستم منPhotosystem II - فتوسیستم 2Light harvesting complex - مجتمع برداشت نورReaction centre - مرکز واکنشAbsorption cross section - مقطع جذب
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The fluorescence emission spectrum of Synechocystis sp. PPC6803 cells, at room temperature, displays: i) significant bandshape variations when collected under open (F0) and closed (FM) Photosystem II reaction centre conditions; ii) a marked dependence on the excitation wavelength both under F0 and FM conditions, due to the enhancement of phycobilisomes (PBS) emission upon their direct excitation. As a consequence: iii) the ratio of the variable and maximal fluorescence (FV/FM), that is a commonly employed indicator of the maximal photochemical quantum efficiency of PSII (Φpc, PSII), displays a significant dependency on both the excitation and the emission (detection) wavelength; iv) the FV/FM excitation/emission wavelength dependency is due, primarily, to the overlap of PSII emission with that of supercomplexes showing negligible changes in quantum yield upon trap closure, i.e. PSI and a PBS fraction which is incapable to transfer the excitation energy efficiently to core complexes. v) The contribution to the cellular emission and the relative absorption-cross section of PSII, PSI and uncoupled PBS are extracted using a spectral decomposition strategy. It is concluded that vi) Φpc, PSII is generally underestimated from the FV/FM measurements in this organism and, the degree of the estimation bias, which can exceed 50%, depends on the measurement conditions. Spectral modelling based on the decomposed emission/cross-section profiles were extended to other processes typically monitored from steady-state fluorescence measurements, in the presence of an actinic illumination, in particular non-photochemical quenching. It is suggested that vii) the quenching extent is generally underestimated in analogy to FV/FM but that viii) the location of quenching sites can be discriminated based on the combined excitation/emission spectral analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 11, November 2018, Pages 1207-1222
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 11, November 2018, Pages 1207-1222
نویسندگان
William Remelli, Stefano Santabarbara,