Isolation and partial characterization of phenol oxidases from Mangifera indica L. sap (latex)

▶ Sap aqueous phase contains low protein but exhibits high specific activities for phenol oxidases. ▶ The aqueous phase of sap was subjected to ion-exchange chromatography on DEAE-Sephacel. ▶ The bound protein was separated into peroxidase polyphenol oxidase and laccase. ▶ Peroxidase and polyphenol oxidase showed stability up to 70 °C while laccase had 60 °C. ▶ Thus, mango sap a by-product in mango industry is a good source of these phenol oxidases.