Cytotoxicity and mutagenicity evaluation of gamma radiation and hydrogen peroxide treated textile effluents using bioassays

Cytotoxicity and mutagenicity of textile effluents, treated by gamma radiation in combination hydrogen peroxide was investigated. The Allium cepa, heamolytic, brine shrimp bioassays were used for cytotoxicity evaluation, whereas mutagenicity was tested using Ames tests. Textile effluents were irradiated to the gamma radiation absorbed doses of 5 kGy, 10 kGy and 15 kGy in combination with 20 mM hydrogen peroxide. Before treatment, textile effluents showed a significant cytotoxicity and mutagenicity signs and reduced significantly after treatment. A. cepa showed reduction in cytotoxicity 50%, whereas 56-59% in case of heamolytic test and up 93% reduction in cytotoxicity was recorded by brine shrimp assays. The mutagenicity of gamma radiation treated effluents reduced up to 59% and 54% in case of TA98 and TA100, respectively. Results revealed that the gamma radiation in combination with hydrogen peroxide can be implemented for the detoxification of textile effluents.