Pumpkin oil cake protein isolate films as potential gas barrier coating

► Pumpkin oil cake protein isolate (PuOC PI) films could be formed in the range of pH, except at pH = 4-8. ► The pH of film-forming solutions influenced all examined film's characteristics. ► The amount of added glycerol significantly affected TS, EB, and film solubility. ► Films prepared with 0.4 g glycerol/g PI showed excellent barrier for O2, N2, CO2, and air. ► Obtained films have improved EB and gas permeability characteristics compared to PuOC films.