کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10450158 | 918347 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reactive oxygen species contribute to neuropathic pain and locomotor dysfunction via activation of CamKII in remote segments following spinal cord contusion injury in rats
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
In this study, we examined whether blocking spinal cord injury (SCI)-induced increases in reactive oxygen species (ROS) by a ROS scavenger would attenuate below-level central neuropathic pain and promote recovery of locomotion. Rats with T10 SCI developed mechanical allodynia in both hind paws and overproduction of ROS, as assayed by Dhet intensity, in neurons in the lumbar 4/5 dorsal horn (âPÂ <Â 0.05). To scavenge ROS, phenyl-N-tert-butylnitrone (PBN, a ROS scavenger) was administered immediately after SCI and for 7 consecutive days (early treatment) by either intrathecal (it; 1 and 3Â mg) or systemic (ip; 10, 50 and 100Â mg) injections. In addition, the high doses of it (3Â mg) or ip (100Â mg) injections were performed at 35Â days (delayed treatment) after SCI. High doses of PBN (ip, 100Â mg, and it, 3Â mg) significantly attenuated mechanical allodynia in both hind paws at both early and delayed treatments, respectively (âPÂ <Â 0.05). The abnormal hyperexcitability of wide dynamic range neurons after SCI was significantly attenuated by both early and delayed PBN treatment (âPÂ <Â 0.05). Early PBN treatment (100Â mg, ip, and 3Â mg, it) attenuated overproduction of ROS in neurons in the lumbar 4/5 dorsal horn. In addition, it and ip t-BOOH (ROS donor) treatment dose-dependently produced mechanical allodynia in both hind paws (âPÂ <Â 0.05). Both SCI and t-BOOH treatment groups showed significantly increased phospho-CamKII (pCamKII) expression in neurons and KN-93 (an inhibitor of pCamKII) significantly attenuated mechanical allodynia (âPÂ <Â 0.05). In addition, high doses of PBN significantly promoted the recovery of locomotion (âPÂ <Â 0.05). In conclusion, the present data suggest that overproduction of ROS contribute to sensory and motor abnormalities in remote segments below the lesion after thoracic SCI.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: PAIN® - Volume 154, Issue 9, September 2013, Pages 1699-1708
Journal: PAIN® - Volume 154, Issue 9, September 2013, Pages 1699-1708
نویسندگان
Young S. Gwak, Shayne E. Hassler, Claire E. Hulsebosch,