کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10451169 | 918391 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The pronociceptive effect of proteinase-activated receptor-4 stimulation in rat knee joints is dependent on mast cell activation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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![عکس صفحه اول مقاله: The pronociceptive effect of proteinase-activated receptor-4 stimulation in rat knee joints is dependent on mast cell activation The pronociceptive effect of proteinase-activated receptor-4 stimulation in rat knee joints is dependent on mast cell activation](/preview/png/10451169.png)
چکیده انگلیسی
Proteinase-activated receptor-4 (PAR4) is a G-protein-coupled receptor activated by serine proteinases released during tissue repair and inflammation. We have previously shown that PAR4 activation sensitises articular primary afferents leading to joint pain. This study examined whether mast cells contribute to this PAR4-induced sensitisation and consequent heightened pain behaviour. The expression of PAR4 on synovial mast cells was confirmed with immunofluorescent staining of rat knee joint sections. Electrophysiological recordings were made from joint primary afferents in male Wistar rats during both nonnoxious and noxious rotations of the knee. Afferent firing rate was recorded for 15 minutes after close intra-arterial injection of 10â9 to 10â5 mol of the PAR4 activating peptide, AYPGKF-NH2, or the inactive peptide, YAPGKF-NH2 (100-μl bolus). Rats were either naive or pretreated with the mast cell stabilise, cromolyn (20 mg/kg). Mechanical withdrawal thresholds were determined using a dynamic planter aesthesiometer and weight bearing determined using an incapacitance tester. These behavioural measurements were taken before and after intra-articular AYPGKF-NH2, or the inactive peptide, YAPGKF-NH2 (100 μg). Local administration of AYPGKF-NH2 caused a significant increase in joint primary afferent firing rate and pain behaviour compared with the control peptide YAPGKF-NH2. These effects were blocked by pretreatment with cromolyn. These data reveal that PAR4 is expressed on synovial mast cells and the activation of PAR4 has a pronociceptive effect that is dependent on mast cell activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: PAIN® - Volume 152, Issue 2, February 2011, Pages 354-360
Journal: PAIN® - Volume 152, Issue 2, February 2011, Pages 354-360
نویسندگان
Fiona A. Russell, Shu Zhan, Aline Dumas, Stéphanie Lagarde, Marc Pouliot, Jason J. McDougall,