Physicochemical and functional properties of the protein isolate and major fractions prepared from Akebia trifoliata var. australis seed

► Albumin and glutelin were major protein fractions in Akebia trifoliata var. australis seed. ► The subunits of seed proteins showed molecular weights ranging from 12.0 to 50.0 kDa. ► Albumin showed the highest solubility, surface hydrophobicity and emulsifying capacity. ► A similar secondary composition was shown in A. trifoliata var. australis seed protein isolate and glutelin.