کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10552775 | 967600 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spectrophotometric method for quantification of kahweol in coffee
ترجمه فارسی عنوان
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کلمات کلیدی
DABACNMTBEglacial acetic acid - اسید استیک یخبندانValidation - اعتبار سنجیHAC - اینجاFood analysis - تجزیه و تحلیل مواد غذاییFood composition - ترکیب مواد غذاییCoffee adulteration - تقلب قهوهDiterpenes - دیترپنسCoffee oil - روغن قهوهmethyl tert-Butyl ether - متیل ترتل-بوتیل اترRegulatory issues - مسائل نظارتیCoffea canephora - کافئا کانفوراCoffea arabica - کافیه عربیquality control - کنترل کیفیت
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The diterpene kahweol, a component of the unsaponifiable matter of coffee oil, has anticarcinogenic and antioxidant properties. Kahweol is specific to Coffea arabica, so it can be used to discriminate between coffee species. Chromatographic or infrared techniques are usually required for the evaluation of kahweol. The objective of this study was to develop a methodology to quantify kahweol based in colorimetric reactions and spectrophotometric measurements. The best extraction conditions were achieved by direct saponification of roasted and ground coffees, extraction with MTBE, cleaning the extract with water, reaction of the extract with KI, dilution with HAc 50% and absorbance analysis at 620 nm for quantification. This method demonstrated good precision (RSD below 5%) considering different extractions and intraday repeatability. Linearity was also observed (R2 = 0.996, p â¤Â 0.05), with low limits of detection (5.16 mg 100 gâ1) and quantification (17.2 mg 100 gâ1). Kahweol contents were assessed at similar levels to those obtained using a standard HPLC methodology, and a good recovery was observed (116%).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 31, Issue 1, August 2013, Pages 137-143
Journal: Journal of Food Composition and Analysis - Volume 31, Issue 1, August 2013, Pages 137-143
نویسندگان
Rafael Carlos Eloy Dias, Sandriel Trindade Alves, Marta de Toledo Benassi,