کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10585792 | 981379 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced reactivity of Lys182 explains the limited efficacy of biogenic amines in preventing the inactivation of glucose-6-phosphate dehydrogenase by methylglyoxal
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کلمات کلیدی
SPEG6PDPDBG6PAGEsMEADl-carnosine - l-کارنوزینLC–MS - LC-MSSpermine - اسپرمSpermidine - اسپرمیدینFormic acid - اسید فرمیکCAR - ماشینMethylglyoxal - متیل گلی اکسالadvanced glycation end-products - محصولات نهایی گلیسایی پیشرفتهMaillard reaction - واکنش میلاردProtein Data Bank - پروتئین بانک اطلاعاتیGlucose 6-phosphate - گلوکز 6-فسفاتglucose 6-phosphate dehydrogenase - گلوکز 6-فسفات دهیدروژناز
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study examines the inactivation of the enzyme glucose 6-phosphate dehydrogenase (G6PD) by methylglyoxal (MG) and the eventual protection exerted by endogenous amines. To determine the protective effect of amines, the rate constant of the reaction of MG with the amino group of N-α-acetyl-lysine, carnosine, spermine and spermidine was measured at pH 7.4, and the behavior of endogenous amines was analyzed on the basis of quantum chemical reactivity descriptors. A 63% reduction in the enzyme activity was found upon incubation of G6PD with MG at pH 7.4. The inactivation of G6PD was even larger when the pH was increased to 9.4, revealing a weak protective effect by the amines. The results suggest that some basic residues of G6PD exhibit an anomalous reactivity, which likely reflects a shift in the standard pKa value due to the local environment in the enzyme. Under the experimental conditions used in the assays, this hypothesis was corroborated by mass spectrometry analysis, which points out that modification of Lys182 in the binding site is responsible for the inactivation of G6PD by MG. These results emphasize the need to search for more effective antiglycating agents, which can compete with basic amino acid residues possessing enhanced reactivity in proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 5, 1 March 2011, Pages 1613-1622
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 5, 1 March 2011, Pages 1613-1622
نویسندگان
Patricio Flores-Morales, Claudio Diema, Marta Vilaseca, Joan Estelrich, F. Javier Luque, Soledad Gutiérrez-Oliva, Alejandro Toro-Labbé, Eduardo Silva,