کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10587480 | 981430 | 2011 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of the placement and total charge of the basic amino acid clusters on antibacterial organism selectivity and potency
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کلمات کلیدی
6-aminohexanoic acidTICOICPoPCITCMICPOPGβAlaAMP1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt)1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine - 1-پالمیتویل-2-اولئویل-اسن-گلیسرو-3-فسفوشولینUnnatural amino acids - آمینو اسیدهای غیر طبیعیAhx - آهکسStaphylococcus aureus - استافیلوکوک اورئوسGamma aminobutyric acid - اسید گاما آمینو بوتیریکlarge unilamellar vesicles - بزرگ کیسه های بدون انعطاف پذیرQSAR - بزرگسال Minimum inhibitory concentration - حداقل غلظت مهاریQuantitative structure–activity relationship - رابطه ساختاری و فعالیت کمیcircular dichroism - رنگ تابی دورانیSalmonella Typhimurium - سالمونلا تیفی موریومCD spectroscopy - طیف سنجی سی دیFluorescence spectroscopy - فوتولومینسانس یا فلوئورسانس یا فسفرسانسLUV - لووSUV - ماشین شاسی بلندCalcein leakage - نشت کلسینAntimicrobial peptide - پپتیدهای ضدمیکروبیAntimicrobial peptides - پپتیدهای پادمیکرب یا آنتیمایکروبیال پپتایدIsothermal titration calorimetry - کالری سنجی تیتاسیون ایزوترمالsmall unilamellar vesicles - کیسه های کوچک کوچکGABA - گابا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Extensive circular dichroism, isothermal titration calorimetry and induced calcein leakage studies were conducted on a series of antimicrobial peptides (AMPs), with a varying number of Lys residues located at either the C-terminus or the N-terminus to gain insight into their effect on the mechanisms of binding with zwitterionic and anionic membrane model systems. Different CD spectra were observed for these AMPs in the presence of zwitterionic DPC and anionic SDS micelles indicating that they adopt different conformations on binding to the surfaces of zwitterionic and anionic membrane models. Different CD spectra were observed for these AMPs in the presence of zwitterionic POPC and anionic mixed 4:1 POPC/POPG LUVs and SUVs, indicating that they adopt very different conformations on interaction with these two types of LUVs and SUVs. In addition, ITC and calcein leakage data indicated that all the AMPs studied interact via very different mechanisms with anionic and zwitterionic LUVs. ITC data suggest these peptides interact primarily with the surface of zwitterionic LUVs while they insert into and form pores in anionic LUVs. CD studies indicated that these compounds adopt different conformations depending on the ratio of POPC to POPG lipids present in the liposome. There are detectable spectroscopic and thermodynamic differences between how each of these AMPs interacts with membranes, that is position and total charge density defines how these AMPs interact with specific membrane models and thus partially explain the resulting diversity of antibacterial activity of these compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 23, 1 December 2011, Pages 7008-7022
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 23, 1 December 2011, Pages 7008-7022
نویسندگان
Amanda L. Russell, Anne M. Spuches, Brittany C. Williams, Divakaramenon Venugopal, David Klapper, Antoine H. Srouji, Rickey P. Hicks,