Branching pattern of gluco-oligosaccharides and 1.5 kDa dextran grafted by the α-1,2 branching sucrase GBD-CD2

► GBD-CD2 is an α-1,2 branching sucrase engineered from DSR-E dextransucrase ► New poly-branched α-1,2 gluco-oligosaccharides were synthesized ► Branching occurring onto vicinal α-1,6 linked glucosyl units was first demonstrated ► 1.5 kDa dextran was shown to be glucosylated at random ► A vast panel of structures showing dietary properties can thus be obtained.