Expression of lactoperoxidase in differentiated mouse colon epithelial cells

► Glutathione peroxidase 1/2 double-KO mice are strain-dependently prone to ileocolitis. ► 129 strain mice have higher lactoperoxidase (Lpo) gene expression than B6 in colon epithelium. ► Lpo DNA is more methylated in 129 than B6, DKO than WT, and mature than young mice. ► Lpo DNA methylation does not result from inflammation nor lead to gene expression. ► Only the full-length variant of colon Lpo mRNA produces an active enzyme.