Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts

► Macroautophagy plays an important role in the uptake of lipofuscin into lysosomes. ► Lysosomes are not required for the formation of fluorescent lipofuscin. ► Lipofuscin accumulates in the cytosolic volume after inhibition of macroautophagy. ► Lipofuscin accumulates in the cytosol after inhibition of lysosomal activity. ► A correlation between cytosolic lipofuscin and cellular viability was shown.