کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739354 | 1046872 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ESR spin trapping of a carbon-centered free radical from agonist-stimulated human platelets
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کلمات کلیدی
PBNAACOCF3PGG2Prostaglandin G2PGHSPGH2DMPOGSNOcPLA2ESRArachidonic acid - اسید آراشیدونیکElectron spin resonance - رزونانس اسپین الکترونcytosolic phospholipase A2 - فسفولیپاز A2 سیتوزولHPETE - هتلیPeroxides - پراکسید هاProstaglandin H synthase - پروستاگلاندین H سنتازProstaglandin H2 - پروستاگلاندین H2Platelets - پلاکت ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: ESR spin trapping of a carbon-centered free radical from agonist-stimulated human platelets ESR spin trapping of a carbon-centered free radical from agonist-stimulated human platelets](/preview/png/10739354.png)
چکیده انگلیسی
Several free radical intermediates formed during synthesis of prostaglandin H synthase (PGHS) catalyze the biosynthesis of prostaglandins from arachidonic acid (AA). We attempted to directly detect free radical intermediates of PGHS in cells. Studies were carried out using human platelets, which possess significant PGHS activity. Electron spin resonance (ESR) spectra showed a g = 2.005 signal radical, which was formed by the incubation of collagen, thrombin, AA, and a variety of peroxides with human platelets. The ESR spectra obtained using 5,5-dimethyl-1 pyrroline N-oxide (DMPO) and α-phenyl N-tert.-butylnitron (PBN) were typical of an immobilized nitroxide. Extensive Pronase digestion of both the DMPO and PBN adducts allowed us to deduce that it was a carbon-centered radical. The formation of this radical was inhibited by potassium cyanide and by desferroxamine. Peroxides stimulated formation of the g = 2.005 signal radical and inhibited platelet aggregation induced by AA. PGHS cosubstrates increased the intensity of the radical signal but inhibited platelet aggregation induced by AA. Both S-nitro-L-glutathione and reduced glutathione quenched the g = 2.005 radical but could not restore platelet aggregatory activity. These results suggest that the carbon-centered radical is a self-destructing free radical formed during peroxide-mediated deactivation of PGHS in human platelets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 2, 15 July 2005, Pages 237-248
Journal: Free Radical Biology and Medicine - Volume 39, Issue 2, 15 July 2005, Pages 237-248
نویسندگان
Duen-Suey Chou, George Hsiao, Ming-Yi Shen, Yan-Jyu Tsai, Tzeng-Fu Chen, Joen-Rong Sheu,