کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739576 | 1046881 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine
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کلمات کلیدی
EPO3,5-dichlorotyrosinePBS containing Tween 20TPBSNaYBRY3-BromotyrosineHOClTFAKLHDIYPBSEDCLPS1-ethyl-3-(3-dimethylaminopropyl)carbodiimide - 1-اتیل-3- (3-dimethylaminopropyl) carbodiimide3-chlorotyrosine - 3-کلروتیروسینBSA - BSAMPO - DFObovine serum albumin - آلبومین سرم گاوeosinophil peroxidase - ائوزینوفیل پراکسیدازTrifluoroacetic acid - اسید TrifluoroaceticHypochloric acid - اسید هیپوکلریکinflammation - التهاب( توروم) sodium dodecyl sulfate–polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدBromotyrosine - بروموتیروزینEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاTyrosine - تیروزینDityrosine - دیتروستینFree radicals - رادیکال آزادMass spectrometry - طیف سنجی جرمیPhosphate buffered saline - فسفات بافر شورLow-density lipoprotein - لیپوپروتئین کم چگالی یا الدیال LDL - لیپوپروتئین کم چگالی(کلسترول بد)lipopolysaccharide - لیپوپلی ساکاریدmyeloperoxidase - میلوپراکسیداز Antibody - پادتَن یا آنتیبادیliquid chromatography - کروماتوگرافی مایعChlorotyrosine - کلروتیروزینkeyhole limpet hemocyanin - کلم هول limpet هموسیانین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine](/preview/png/10739576.png)
چکیده انگلیسی
During inhalation of allergens and experimental sepsis, formation of brominated tyrosine has been reported. In this study, we first examined the immunogenicity of brominated protein prepared by treatment of N-bromosuccinimide (NBS). The immunized serum obtained reacted with brominated bovine serum albumin (BSA). The NBS dose-dependent formation of immunoreactivity, which was estimated by enzyme-linked immunosorbent assay, was observed, and the increase coincided with 3,5-dibromotyrosine (DiBrY) formation in the modified BSA, which was chemically determined by liquid chromatography/quadrupole tandem mass spectrometry (LC/MS/MS). Second, by use of immunized mice, monoclonal antibodies to the brominated one were prepared. The two established novel monoclonal antibodies obtained from the immunized mice reacted with DiBrY, 3,5-dichlorotyrosine (DiClY), and 3,5-diiodotyrosine (DiIY). Moreover, 3,5-dihalo-4-hydroxybenzoic acids (3,5-dichloro-4-hydroxybenzoic acid and 3,5-dibromo-4-hydroxybenzoic acid) were recognized by these antibodies. These results suggest that dihalogenated tyrosines (DiBrY, DiClY, and DiIY) are the epitopes. Lastly, we used the antibody in an immunohistochemical study. Lipopolysaccharide (LPS) was intraperitoneally administered to mice, and livers were removed. Positive staining of LPS-treated mouse liver tissues by both the anti-dihalotyrosine antibody and anti-myeloperoxidase antibody was estimated, suggesting that inflammatory tissue damage induces the formation of dihalotyrosine in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 1, 1 January 2005, Pages 24-31
Journal: Free Radical Biology and Medicine - Volume 38, Issue 1, 1 January 2005, Pages 24-31
نویسندگان
Yoji Kato, Yoshichika Kawai, Hiroshi Morinaga, Hajime Kondo, Natsuko Dozaki, Noritoshi Kitamoto, Toshihiko Osawa,