کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10748134 | 1050264 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Significant reduction in allergenicity of ovalbumin from chicken egg white following treatment with ascidian viscera N-acetylglucosaminidase
ترجمه فارسی عنوان
کاهش قابل ملاحظه آلرژیزاسیون تخم بلومین از سفید تخم مرغ پس از درمان با ویسکره آسکی دی متیل گلوکوزامینیداز
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کلمات کلیدی
IC50GlcNAcAVEN-acetylglucosaminidase - N-استیل گلوکوزامینیدازAllergenicity - آلرژن بودنAscidian - اسکیدینsodium dodecyl sulfate–polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدOvalbumin - اوبلبومینMannose - مانوزMan - مردN-acetylglucosamine - نیتستیگلوکوزامینhalf-maximal inhibitory concentration - نیمه حداکثر غلظت مهاریHPLC - کروماتوگرافی مایعی کاراhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراIoA - یوآآ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Ovalbumin (OA) is the most abundant ingredient of chicken egg-white allergenic proteins. In the present study we investigated the possibility of reducing OA allergenicity by treatment with a natural protein exhibiting N-acetylglucosaminidase (NA) activity. Ascidian is cultivated as a food resource in northeast Asia. The ascidian viscera NA (AVNA) with almost no other exoglycosidases or proteolytic enzymes was isolated by applying size-exclusion chromatography to a protein precipitate of ascidian viscera. Intact OA was mixed with AVNA containing 0.2, 1.0, and 5.0 Units of NA. Anion-exchange chromatography was then used to isolate OA from AVNA-treated OA. The electrophoretic patterns and N-glycans of each isolated OA from AVNA-treated OA (iOA) were analyzed, and the terminal N-acetylglucosamines of iOA were selectively cleaved with no other degradation occurring. A competitive indirect enzyme-linked immunosorbent assay using rabbit anti-OA sera was performed to investigate the allergenicity of iOA, which was found to be significantly reduced depending on the increased NA activity compared to that of intact OA. These results indicate that OA allergenicity was reduced using a simple and mild treatment process with AVNA, and suggest that ascidian NA is an efficient natural protein for reducing the allergenicity of OA without requiring the use of harsh physical treatments or chemical conjugation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 475, Issue 1, 17 June 2016, Pages 107-112
Journal: Biochemical and Biophysical Research Communications - Volume 475, Issue 1, 17 June 2016, Pages 107-112
نویسندگان
Hye Seong Hwang, Heajin Park, Jihye Kim, Jai Yeon Choi, Young Kwang Lee, Ho-Young Park, Hee-Don Choi, Ha Hyung Kim,