کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749494 | 1050291 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heparanase augments inflammatory chemokine production from colorectal carcinoma cell lines
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کلمات کلیدی
PBSmAbFCSHPSEBSA - BSAbovine serum albumin - آلبومین سرم گاوMonoclonal antibody - آنتی بادی مونوکلونالELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاcolorectal carcinoma - سرطان روده بزرگ و مقعدfetal calf serum - سرم گوساله جنینPhosphate buffered saline - فسفات بافر شورHeparan sulfate - هپاران سولفاتHeparanase - هپارنازChemokine - کموکاین یا کموکین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
To explore possible roles of heparanase in cancer-host crosstalk, we examined whether heparanase influences expression of inflammatory chemokines in colorectal cancer cells. Murine colorectal carcinoma cells incubated with heparanase upregulated MCP-1, KC, and RANTES genes and released MCP-1 and KC proteins. Heparanase-dependent production of IL-8 was detected in two human colorectal carcinoma cell lines. Addition of a heparanase inhibitor Heparastatin (SF4) did not influence MCP-1 production, while both latent and mature forms of heparanase augmented MCP-1 release, suggesting that heparanase catalytic activity was dispensable for MCP-1 production. In contrast, addition of heparin to the medium suppressed MCP-1 release in a dose-dependent manner. Similarly, targeted suppression of Ext1 by RNAi significantly suppressed cell surface expression of heparan sulfate and MCP-1 production in colon 26Â cells. Taken together, it is concluded that colon 26Â cells transduce the heparanase-mediated signal through heparan sulfate binding. We propose a novel function for heparanase independent of its endoglycosidase activity, namely as a stimulant for chemokine production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 469, Issue 4, 22 January 2016, Pages 878-883
Journal: Biochemical and Biophysical Research Communications - Volume 469, Issue 4, 22 January 2016, Pages 878-883
نویسندگان
Naoki Tsunekawa, Nobuaki Higashi, Yusuke Kogane, Michihiko Waki, Hiroaki Shida, Yoshio Nishimura, Hayamitsu Adachi, Motowo Nakajima, Tatsuro Irimura,