کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10753621 | 1050344 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trans-Golgi protein p230/golgin-245 is involved in phagophore formation
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کلمات کلیدی
LC3AP2EBSSTGNMACF1siRNA - siRNAAutophagy - اتوفاژیImmunochemistry - ایمونوشیمیKnock-down - دست کشیدنEarle’s Balanced Salt Solution - راه حل نمکی متعادل ارلTrans-Golgi network - شبکه Trans-Goljiendoplasmic reticulum - شبکه آندوپلاسمی Plasma membrane - غشای پلاسماMembrane trafficking - قاچاق غشاءmicrotubule-associated protein 1 light chain 3 - پروتئین مرتبط با میکروتوبول 1 زنجیره سبک 3Golgi - گلجی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
p230/golgin-245 is a trans-Golgi coiled-coil protein that is known to participate in regulatory transport from the trans-Golgi network (TGN) to the cell surface. We investigated the role of p230 and its interacting protein, microtubule actin crosslinking protein 1 (MACF1), in amino acid starvation-induced membrane transport. p230 or MACF1 knock-down (KD) cells failed to increase the autophagic flow rate and the number of microtubule-associated protein 1 light chain 3 (LC3)-positive puncta under starvation conditions. Loss of p230 or MACF1 impaired mAtg9 recruitment to peripheral phagophores from the TGN, which was observed in the early step of autophagosome formation. Overexpression of the p230-binding domain of MACF1 resulted in the inhibition of mAtg9 trafficking in starvation conditions as in p230-KD or MACF1-KD cells. These results indicate that p230 and MACF1 cooperatively play an important role in the formation of phagophore through starvation-induced transport of mAtg9-containing membranes from the TGN. In addition, p230 itself was detected in autophagosomes/autolysosome with p62 or LC3 during autophagosome biogenesis. Thus, p230 is an important molecule in phagophore formation, although it remains unclear whether p230 has any role in late steps of autophagy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 456, Issue 1, 2 January 2015, Pages 275-281
Journal: Biochemical and Biophysical Research Communications - Volume 456, Issue 1, 2 January 2015, Pages 275-281
نویسندگان
Miwa Sohda, Yoshio Misumi, Shigenori Ogata, Shotaro Sakisaka, Shinichi Hirose, Yukio Ikehara, Kimimitsu Oda,