کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754087 | 1050351 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
IL-27 affects helper T cell responses via regulation of PGE2 production by macrophages
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
COXMicrosomal prostaglandin E2 synthase-1H-PGDSmPGES-1IL-27BMDMCOX-2APCsAntigen presenting cells - آنتیژن ارائه سلولcyclooxygenase - آنزیم سیکلواکسیژنازhelper T cells - سلول T کمکیDendritic cell - سلول دندریتیکConditioned medium - شرایط محیطیMacrophage - ماکروفاژ bone marrow-derived macrophage - ماکروفاژ حاصل از مغز استخوانbone marrow - مغز استخوانhematopoietic prostaglandin D synthase - هموگلوبین پروستاگلاندین D سنتازprostaglandin - پروستاگلاندینها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
IL-27 is a heterodimeric cytokine that regulates both innate and adaptive immunity. The immunosuppressive effect of IL-27 largely depends on induction of IL-10-producing Tr1 cells. To date, however, effects of IL-27 on regulation of immune responses via mediators other than cytokines remain poorly understood. To address this issue, we examined immunoregulatory effects of conditional medium of bone marrow-derived macrophages (BMDMs) from WSX-1 (IL-27Rα)-deficient mice and found enhanced IFN-γ and IL-17A secretion by CD4+ T cells as compared with that of control BMDMs. We then found that PGE2 production and COX-2 expression by BMDMs from WSX-1-deficient mice was increased compared to control macrophages in response to LPS. The enhanced production of IFN-γ and IL-17A was abolished by EP2 and EP4 antagonists, demonstrating PGE2 was responsible for enhanced cytokine production. Murine WSX-1-expressing Raw264.7 cells (mWSX-1-Raw264.7) showed phosphorylation of both STAT1 and STAT3 in response to IL-27 and produced less amounts of PGE2 and COX-2 compared to parental RAW264.7 cells. STAT1 knockdown in parental RAW264.7 cells and STAT1-deficiency in BMDMs showed higher COX-2 expression than their respective control cells. Collectively, our result indicated that IL-27/WSX-1 regulated PGE2 secretion via STAT1-COX-2 pathway in macrophages and affected helper T cell response in a PGE2-mediated fashion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 451, Issue 2, 22 August 2014, Pages 215-221
Journal: Biochemical and Biophysical Research Communications - Volume 451, Issue 2, 22 August 2014, Pages 215-221
نویسندگان
Yayoi Sato, Hiromitsu Hara, Toshiaki Okuno, Naoko Ozaki, Shinobu Suzuki, Takehiko Yokomizo, Tsuneyasu Kaisho, Hiroki Yoshida,