کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754574 | 1050357 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bufotalin-induced apoptosis in osteoblastoma cells is associated with endoplasmic reticulum stress activation
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کلمات کلیدی
shRNAUPRIRE1eIF2αATF6double-stranded RNA-activated protein kinase (PKR)-like ER kinaseC/EBP homologous protein - C / EBP پروتئین همولوگshort hairpin RNA - RNA موی سر کوتاهinositol-requiring enzyme 1 - آنزیم مورد نیاز inositol 1Osteoblastoma - استئوبلاستوماER stress - استرس استCHOP - تکه کردنendoplasmic reticulum - شبکه آندوپلاسمی activating transcription factor 6 - فعال کردن عامل رونویسی 6Unfolded protein response - پاسخ پروتئین آشکارPERK - پرک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The search for novel and more efficient chemo-agents against malignant osteoblastoma is important. In this study, we examined the potential anti-osteoblastoma function of bufotalin, and studied the underlying mechanisms. Our results showed that bufotalin induced osteoblastoma cell death and apoptosis in dose- and time-dependent manners. Further, bufotalin induced endoplasmic reticulum (ER) stress activation in osteoblastoma cells, the latter was detected by the induction of C/EBP homologous protein (CHOP), phosphorylation of inositol-requiring enzyme 1 (IRE1) and PKR-like endoplasmic reticulum kinase (PERK), as well as caspase-12 activation. Conversely, the ER stress inhibitor salubrinal, the caspase-12 inhibitor z-ATAD-fmk as well as CHOP depletion by shRNA significantly inhibited bufotalin-induced osteoblastoma cell death and apoptosis. Finally, by using a mice xenograft model, we demonstrated that bufotalin inhibited U2OS osteoblastoma cell growth in vivo. In summary, our results suggest that ER stress contributes to bufotalin-induced apoptosis in osteoblastoma cells. Bufotalin might be investigated as a novel anti-osteoblastoma agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 451, Issue 1, 15 August 2014, Pages 112-118
Journal: Biochemical and Biophysical Research Communications - Volume 451, Issue 1, 15 August 2014, Pages 112-118
نویسندگان
Yun-Rong Zhu, Yong Xu, Jian-Feng Fang, Feng Zhou, Xiong-Wei Deng, Yun-Qing Zhang,