کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10756996 | 1050390 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Berberine slows cell growth in autosomal dominant polycystic kidney disease cells
ترجمه فارسی عنوان
بربرین باعث کاهش رشد سلول در سلولهای بیماری کلیوی غالب اتوزومال می شود
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کلمات کلیدی
EGFRmTORERKADPKDcAMP - cAMPextracellular-signal-regulated kinases - kineases regulated by extracellular-signal-regulatedCyclic adenosine monophosphate - آدنوزین مونوفسفات CyclicBerberine - بربرینCell proliferation - تکثیر سلولیApoptosis - خزان یاختهایmammalian target of rapamycin - هدف پستانداران رپامایسینEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary monogenic disorder characterized by development and enlargement of kidney cysts that lead to loss of renal function. It is caused by mutations in two genes (PKD1 and PKD2) encoding for polycystin-1 and polycystin-2 proteins which regulate different signals including cAMP, mTOR and EGFR pathways. Abnormal activation of these signals following PC1 or PC2 loss of function causes an increased cell proliferation which is a typical hallmark of this disease. Despite the promising findings obtained in animal models with targeted inhibitors able to reduce cystic cell growth, currently, no specific approved therapy for ADPKD is available. Therefore, the research of new more effective molecules could be crucial for the treatment of this severe pathology. In this regard, we have studied the effect of berberine, an isoquinoline quaternary alkaloid, on cell proliferation and apoptosis in human and mouse ADPKD cystic cell lines. Berberine treatment slows cell proliferation of ADPKD cystic cells in a dose-dependent manner and at high doses (100 μg/mL) it induces cell death in cystic cells as well as in normal kidney tubule cells. However, at 10 μg/mL, berberine reduces cell growth in ADPKD cystic cells only enhancing G0/G1 phase of cell cycle and inhibiting ERK and p70-S6 kinases. Our results indicate that berberine shows a selected antiproliferative activity in cellular models for ADPKD, suggesting that this molecule and similar natural compounds could open new opportunities for the therapy of ADPKD patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 3, 22 November 2013, Pages 668-674
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 3, 22 November 2013, Pages 668-674
نویسندگان
Anna Bonon, Alessandra Mangolini, Paolo Pinton, Laura del Senno, Gianluca Aguiari,