کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10758062 | 1050403 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Targeted disruption of fad24, a regulator of adipogenesis, causes pre-implantation embryonic lethality due to the growth defect at the blastocyst stage
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کلمات کلیدی
PBSHBO1DAPINEO4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولAdipogenesis - آدیپوژنزFAD - بدblastocyst - بلاستوسیستthymidine kinase - تیمیدین کینازEmbryonic development - رشد رویانembryonic day - روز جنینیNOC - شبPhosphate buffered saline - فسفات بافر شورIn vitro fertilization - لقاح در خارج از بدن یا آیویافIVF - لقاح مصنوعی Embryonic lethality - مرگ و میر جنینneomycin resistance - مقاومت نئومیسینMorula - مورولاpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In previous studies, we identified a novel gene, factor for adipocyte differentiation 24 (fad24), which plays an important role during the early stages of adipogenesis in mouse 3T3-L1 cells. Moreover, overexpression of fad24 increased the number of smaller adipocytes in white adipose tissue and improved glucose metabolic activity in mice, thus indicating that fad24 functions as a regulator of adipogenesis in vivo. However, the physiological roles of fad24 in vivo are largely unknown. In this study, we attempted to generate fad24-deficient mice by gene targeting. No fad24-null mutants were recovered after embryonic day 9.5 (E9.5). Although fad24-null embryos were detected in an expected Mendelian ratio of genotypes at E3.5, none of the homozygous mutants developed into blastocysts. In vitro culture experiments revealed that fad24-null embryos develop normally to the morula stage but acquire growth defects during subsequent stages. The number of nuclei decreased in fad24-deficient morulae compared with that in wild-type ones. These results strongly suggested that fad24 is essential for pre-implantation in embryonic development, particularly for the progression to the blastocyst stage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 438, Issue 2, 23 August 2013, Pages 301-305
Journal: Biochemical and Biophysical Research Communications - Volume 438, Issue 2, 23 August 2013, Pages 301-305
نویسندگان
Natsuki Ochiai, Makoto Nishizuka, Tomomi Miyamoto, Ichiro Miyoshi, Masahito Ikawa, Shigehiro Osada, Masayoshi Imagawa,