کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765427 | 1050592 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NMR characterization of a 264-residue hyperthermostable endo-β-1,3-glucanase
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کلمات کلیدی
DSCHSQCNOESYnuclear magnetic resonance - رزونانس مغناطیسی هستهایNuclear Overhauser Effect SpectroscopY - Spectroscopy اثر Overhauser هسته ایNMR - تشدید مغناطیسی هستهای Laminarin - لامینارینHeteronuclear Single Quantum Correlation - همبستگی کوانتومی تک هسته ای تنهاProtein - پروتئینDifferential scanning calorimetry - کالریمتری روبشی افتراقیCalorimetry - گرماسنجیglycoside hydrolase - گلیکوزید هیدرولاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Insight into the hyperthermostable endo-β-1,3-glucanase pfLamA from Pyrococcus furiosus is obtained by using NMR spectroscopy. pfLamA functions optimally at 104 °C and recently the X-ray structure of pfLamA has been obtained at 20 °C, a temperature at which the enzyme is inactive. In this study, near-complete (>99%) NMR assignments are presented of chemical shifts of pfLamA in presence and absence of calcium at 62 °C, a temperature at which the enzyme is biologically active. The protein contains calcium and the effects of calcium on the protein are assessed. Calcium binding results in relatively small chemical shift changes in a region distant from the active site of pfLamA and thus causes only minor conformational modifications. Removal of calcium does not significantly alter the denaturation temperature of pfLamA, implying that calcium does not stabilize the enzyme against global unfolding. The data obtained form the basis for elucidation of the molecular origins involved in conformational stability and biological activity of hyperthermophilic endo-β-1,3-glucanases at extreme temperatures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 391, Issue 1, 1 January 2010, Pages 370-375
Journal: Biochemical and Biophysical Research Communications - Volume 391, Issue 1, 1 January 2010, Pages 370-375
نویسندگان
Johannes H. Ippel, Sotirios Koutsopoulos, Sanne M. Nabuurs, Willem J.H. van Berkel, John van der Oost, Carlo P.M. van Mierlo,