کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767423 | 1050733 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of amino acid residues in the Ah receptor involved in ligand binding
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کلمات کلیدی
AHRTCDDYFPβ-NF3-methylcholanthrene2,3,7,8-Tetrachlorodibenzo-p-dioxin - 2،3،7،8-تترا کلریدیبنزوپتوفان دیوکسینβ-naphthoflavone - β-naftoflavoneAh receptor - آه گیرندهXenobiotics - بیگانه زیست، زنوبیوتیکPAS domain - دامنه PASLigand binding - لیگاند اتصالComputer modeling - مدل سازی کامپیوتریyellow fluorescent protein - پروتئین فلورسنت زردaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The Ah receptor (AhR) is a ligand-activated transcription factor. Five amino acids as candidate amino acids necessary for ligand binding within or near the ligand-binding domain were selected based on their evolutional conservation and their aromatic nature that could interact with xenobiotic ligands. These amino acids were changed to Ala, and the mutated AhRs were subjected to a test of their transactivation activity in HeLa cells. Mutation of Phe318 completely lost its activity whereas other mutations only weakly impaired activity. The Leu-substituted mutant, AhR(Phe318Leu), activated the luciferase activity to the level comparable to wild type in the cells treated with 3-methylcholanthrene (MC) but not at all with β-naphthoflavone (β-NF). Ligand-binding activity of mutants was examined with [3H]MC in vitro. AhR(Phe318Ala) could not bind to [3H]MC. [3H]MC bound by AhR(Phe318Leu) was competed with unlabeled MC but not with β-NF. A structural model of the ligand-binding domain was constructed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 2, 9 March 2007, Pages 396-402
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 2, 9 March 2007, Pages 396-402
نویسندگان
Kenji Goryo, Ai Suzuki, Carlos A. Del Carpio, Kazuhiro Siizaki, Eisuke Kuriyama, Yoshinori Mikami, Koshi Kinoshita, Ken-ichi Yasumoto, Agneta Rannug, Akira Miyamoto, Yoshiaki Fujii-Kuriyama, Kazuhiro Sogawa,