کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768440 | 1050810 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of growth-blocking peptide expression during embryogenesis of the cabbage armyworm
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Regulation of growth-blocking peptide expression during embryogenesis of the cabbage armyworm Regulation of growth-blocking peptide expression during embryogenesis of the cabbage armyworm](/preview/png/10768440.png)
چکیده انگلیسی
Growth-blocking peptide (GBP) is an insect cytokine with diverse biological functions. Northern blot analysis revealed high heterogeneity in the size distribution of GBP mRNAs as well as in the tissues where they are detected. The spatio-temporal transcription pattern is dynamic, especially during embryogenesis. Gel shift assays demonstrated that the cabbage armyworm embryo nuclear extract specifically binds to a 178-bp element, at position +234 to +411 from the transcription start site of the 1.3Â kb GBP transcript, in which two Drosophila Deformed (Dfd) binding sites are repeated in tandem. The specific binding between this element and Dfd was demonstrated using recombinant cabbage armyworm Dfd protein. Silencing the Dfd expression in embryos by treating with Dfd double-stranded RNA did not reduce the expression level of GBP, but ectopic GBP expression was observed in the lateral region of the embryo, suggesting that Dfd could serve as a transcriptional repressor for the GBP gene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 4, 7 October 2005, Pages 1078-1084
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 4, 7 October 2005, Pages 1078-1084
نویسندگان
Seiji Tsuzuki, Shiroh Sekiguchi, Yoichi Hayakawa,