کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768491 | 1050811 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rho-kinase induces association of adducin with the cytoskeleton in platelet activation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We examined whether adducin function is regulated through Rho-kinase after agonist stimulation in platelets. A variety of stimuli such as thrombin, STA2 (a stable analog of TXA2), Ca2+ ionophore, phorbol diester, and shear stress induced phosphorylation of α-adducin at Thr445. Preincubation with the Rho-kinase inhibitor Y-27632 in platelets inhibited agonist-induced phosphorylation of α-adducin. STA2 stimulation led to a redistribution of adducin from Triton-insoluble (high speed) fraction (membrane skeleton) to Triton-insoluble (low speed) fraction (cytoskeleton) and detergent-soluble fraction. Phosphoadducin at Thr445 was selectively isolated in the cytoskeletal fraction, whereas phosphoadducin at Ser726 was mainly present in the Triton-soluble fraction. Y-27632 inhibition of STA2-induced α-adducin phosphorylation at Thr445 inhibited incorporation of α-adducin and spectrin into the platelet cytoskeleton, although Y-27632 did not affect phosphorylation of α-adducin at Ser726. These results suggest that Rho-kinase regulates the association of α-adducin and spectrin with the actin cytoskeleton in platelet activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 2, 1 July 2005, Pages 347-351
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 2, 1 July 2005, Pages 347-351
نویسندگان
Satoshi Tamaru, Tetsu Fukuta, Kozo Kaibuchi, Yoichiro Matsuoka, Hiroshi Shiku, Masakatsu Nishikawa,