کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10769957 1050827 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RNA interference: A mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
RNA interference: A mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells
چکیده انگلیسی
SID-1 is a transmembrane protein that mediates systemic RNA interference in Caenorhabditis elegans. Here we show that the mammalian SID-1 homologue FLJ20174 localizes to the cell membrane of human cells and enhances their uptake of small interfering RNA (siRNA), resulting in increased siRNA-mediated gene silencing efficacy. This is the first demonstration to show that overexpression of a membrane protein enhances siRNA internalization in mammalian cells. This observation raises the possibility of enhancing the efficacy of RNA interference.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 331, Issue 2, 3 June 2005, Pages 459-463
نویسندگان
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