Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95

► This work provides the first glimpse of ion channel clusters at the molecular level. ► The structures show multivalent interactions between Kir2.1 and PSD-95 that drive the self-assembly of complexes. ► A combination of orthogonal structural techniques allows the positioning of high-resolution structures. ► Consistent models for all of the components involved in the formation of the Kir2.1/PSD-95 complexes are shown.