Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump

► Oleic and linoleic acids are active components in Nigella Sativa seeds. ► Oleic and linoleic acids increase the number of static sodium pump units. ► Oleic and linoleic acids enhance ouabain interaction with Na,K-ATPase. ► Oleic and linoleic acids possibly act as Na,K-ATPase chaperons.