Effects of positively charged arginine residues on membrane pore forming activity of Rev-NIS peptide in bacterial cells

► We design two analogs of Rev-NIS, substituted with arginine and serine. ► We investigate the antibacterial property of the peptides. ► All the peptides exert the activity on the bacterial membranes. ► Anal R is especially thought to be the most potent antibacterial peptide. ► The net positive charge of arginine residues is one of the most influential factors.