On the miscibility of cardiolipin with 1,2-diacyl phosphoglycerides: Binary mixtures of dimyristoylphosphatidylethanolamine and tetramyristoylcardiolipin

►Mixing of DMPE and TMCL is non-ideal over the composition range 5-95 mol.%. ►Hydrocarbon chain-melting temperatures and mixture composition are poorly correlated. ►Chain-melting enthalpy is strongly correlated with the mol fraction of TMCL chains. ►Mixtures containing ≥ 10 mol % TMCL form TMCL-like Lc phases upon cooling. ►TMCL inhibits the formation of DMPE-like Lc phases in DMPE-rich mixtures.