کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799351 | 1054318 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the human zinc finger protein 267 promoter: Essential role of nuclear factor Y
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کلمات کلیدی
ECMKLFNF-YHSCsCTFINFγFCSTGFβS-nitroso-N-acetylpenicillamineHEKMMPEMSATNFαKrüppel associated boxC/EBP - C / EBPElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزinterferon γ - اینترفرون γinterleukin - اینترلوکینTransforming growth factor β - تبدیل فاکتور رشد βrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدGene regulation - تنظیم ژنtumor necrosis factor α - تومور نکروز عامل αCCAAT box - جعبه CCAATfetal calf serum - سرم گوساله جنینHepatic stellate cell - سلول ستاره ای کبدیHepatic stellate cells - سلولهای ستارهای کبدیSNAP - ضربه محکم و ناگهانیNuclear factor Y - عامل هسته ای YKrüppel-like factor - عامل کریپال مانندExtracellular matrix - ماتریکس خارج سلولیmatrix metalloproteinase - ماتریکس متالوپروتئینازRace - مسابقهCCAAT/enhancer-binding protein - پروتئین CCAAT / تقویت کنندهPromoter - پروموترKRAB - کراوبhuman embryonic kidney - کلیه جنین انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Liver fibrosis results from an excessive deposition of extracellular matrix proteins secreted by activated hepatic stellate cells (HSCs). The activation process is accompanied by an increased activity of various transcription factors, including zinc finger protein 267 (ZNF267). Recently, ZNF267 has been shown to modulate gene expression and to function as a transcriptional repressor. MMP-10 was identified as a target gene; its gene expression and promoter activity are inhibited by ZNF267, which might promote liver fibrogenesis through diminished matrix degradation. However, the transcriptional regulation of the ZNF267 gene is unknown. In the present study, we have cloned and characterized the human ZNF267 promoter containing a 1.5 kb fragment of the 5â²-flanking region (â1414/+173). The ZNF267 gene has a TATA-less promoter with multiple transcription initiation sites. Analysis of serial 5â²-deletions of luciferase reporter constructs revealed a minimal promoter between â72 and +173 bp. Mutational analysis of putative regulatory elements indicated that a CCAAT box within this region was essential for ZNF267 promoter activity. Electrophoretic mobility shift assays demonstrated that transcription factor nuclear factor Y (NF-Y) bound to the CCAAT box. In co-transfection experiments, NF-YA increased the promoter activity of ZNF267. In conclusion, our results suggest that the binding site for NF-Y is critical for ZNF267 gene regulation and, herewith, the activation of this transcriptional factor may play an important role in the activation process of HSCs and in liver fibrosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1729, Issue 1, 25 May 2005, Pages 14-23
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1729, Issue 1, 25 May 2005, Pages 14-23
نویسندگان
Kanghong Hu, Marina Fink, Matthias Froh, Erwin Gäbele, Claus Hellerbrand, Marcus Mühlbauer, Reiner Wiest, Jürgen Schölmerich, Bernd Schnabl,