کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801048 | 1054725 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cytochrome P450 expression and catalytic activity in coronary arteries and liver of cattle
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
pentoxyresorufin O-depentylasePRODCyPVasoregulation20-HETEERODEDHF20-Hydroxyeicosatetraenoic acid - 20-Hydroxyeicosatetraenoic اسیدethoxyresorufin O-deethylase - اتoxyresorufin O-دی اتیلازArachidonic acid - اسید آراشیدونیکepoxyeicosatrienoic acid - اسید اپوکسی اسیاتریتروئینBROD - برادEET - خوردنCytochrome P450 - سیتوکروم پی۴۵۰Coronary artery - عروق کرونرEndothelium-derived hyperpolarizing factor - فاکتور هیپرپولاریزاسیون حاصل از اندوتلیومNitric oxide - نیتریک اکسیدprostaglandin - پروستاگلاندینهاLiver - کبدCattle - گاو
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
There is increasing evidence that cytochrome P450 (CYP) enzymes are involved not only in the metabolism of xenobiotics, but also in vascular homeostasis. Among the CYP-derived vasoactive agents, special importance is assigned to endogenous products from arachidonic acid (AA). Specifically, the vasodilator epoxyeicosatrienoic acids (EETs), being linked to the CYP 2B, 2C, and 2J subfamilies, and the vasoconstrictor 20-hydroxyeicosatetraenoic acid (20-HETE) connected instead to the CYP 4A subfamily and, to a lesser degree, to isoforms of the CYP 1A and 2E subfamilies. Here, we have examined the occurrence of functional CYP isoforms in the coronary arteries of cattle by RT-PCR with sequence verification, Western immunoblotting, and analysis of distinct catalytic activities with fluorescent substrate probes. Liver tissue was examined comparatively. Coronary tissue expressed mRNA transcripts and immunoreactive proteins belonging to the CYP 1A, 2C, 2E, and 2J subfamilies. Appropriate catalytic activity was ascertained with all these CYP species except 2J. A broader spectrum of CYP enzymes (CYP 1A, 2B, 2C, 2D, 2E, 2J, 3A, 4A subfamilies) was found in liver tissue with catalytic activities exceeding many fold those of coronary tissue. We conclude that bovine coronary arteries are endowed with a full-fledged CYP system with potential for AA-linked vasoregulation through dilator rather than constrictor agents. The same tissue and, to a much larger degree, liver tissue possess the capability of metabolizing xenobiotics via the CYP pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1722, Issue 2, 11 March 2005, Pages 116-123
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1722, Issue 2, 11 March 2005, Pages 116-123
نویسندگان
Emanuela Grasso, Vincenzo Longo, Flavio Coceani, Pier Giovanni Gervasi,