کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801628 | 1055627 | 2016 | 52 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cardiomyocytes from human pluripotent stem cells: From laboratory curiosity to industrial biomedical platform
ترجمه فارسی عنوان
کاردیومیوسیت ها از سلول های بنیادی پلوروپتوژن انسان: از کنجکاوی آزمایشگاهی تا پلت فرم پزشکی
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کلمات کلیدی
Electrophysiology - الکتروفیزیولوژیContractility - انقباضengineered heart tissue - بافت قلب مهندسی شدهCalcium imaging - تصویر برداری کلسیمیHuman induced pluripotent stem cells - سلول های بنیادی تکامل یافته القا شده توسط انسانhuman embryonic stem cells - سلول های بنیادی جنینی انسانDrug screening - غربالگری مواد مخدرCardiomyocytes - قلب و عروقDisease modelling - مدل سازی بیماریMitochondria - میتوکندریا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Cardiomyocytes from human pluripotent stem cells (hPSCs-CMs) could revolutionise biomedicine. Global burden of heart failure will soon reach USD $90bn, while unexpected cardiotoxicity underlies 28% of drug withdrawals. Advances in hPSC isolation, Cas9/CRISPR genome engineering and hPSC-CM differentiation have improved patient care, progressed drugs to clinic and opened a new era in safety pharmacology. Nevertheless, predictive cardiotoxicity using hPSC-CMs contrasts from failure to almost total success. Since this likely relates to cell immaturity, efforts are underway to use biochemical and biophysical cues to improve many of the ~Â 30 structural and functional properties of hPSC-CMs towards those seen in adult CMs. Other developments needed for widespread hPSC-CM utility include subtype specification, cost reduction of large scale differentiation and elimination of the phenotyping bottleneck. This review will consider these factors in the evolution of hPSC-CM technologies, as well as their integration into high content industrial platforms that assess structure, mitochondrial function, electrophysiology, calcium transients and contractility. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 7, Part B, July 2016, Pages 1728-1748
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 7, Part B, July 2016, Pages 1728-1748
نویسندگان
Chris Denning, Viola Borgdorff, James Crutchley, Karl S.A. Firth, Vinoj George, Spandan Kalra, Alexander Kondrashov, Minh Duc Hoang, Diogo Mosqueira, Asha Patel, Ljupcho Prodanov, Divya Rajamohan, William C. Skarnes, James G.W. Smith, Lorraine E. Young,