کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10802052 | 1055653 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human PLacental eXpanded (PLX) mesenchymal-like adherent stromal cells confer neuroprotection to nerve growth factor (NGF)-differentiated PC12 cells exposed to ischemia by secretion of IL-6 and VEGF
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کلمات کلیدی
TBARSMSCIL-6mAbSemaxanibOGDFITCPC12NGFFCS4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl - 4-هیدروکسی 2،2،6،6-تترامتیلیکپیریدین-1-اکسیلROS - ROSHydrogen peroxide - آب اکسیژنهMonoclonal antibody - آنتی بادی مونوکلونالIschemia - ایسکمیinterleukin 6 - اینترلوکین 6Enzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاtwo dimensional - دو بعدیReperfusion - رپرفیوژنhorse serum - سرب اسبfetal calf serum - سرم گوساله جنینTempol - سریعMesenchymal stem cells - سلول های بنیادی مزانشیمیpheochromocytoma cells - سلولهای فئوکروموسیتوماthree dimensional - سه بعدیConditioned medium - شرایط محیطیVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)nerve growth factor - فاکتور رشد عصبfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Neuroprotection - محافظت نورونی یا محافظت از عصبOxygen and glucose deprivation - محرومیت از اکسیژن و گلوکزthiobarbituric acid reactive substances - مواد واکنش پذیر اسید تیوباربیتوریکH2O2 - هیدروژن پراکسیدfluorescence unit - واحد فلورسانسReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Human PLacental eXpanded (PLX) mesenchymal-like adherent stromal cells confer neuroprotection to nerve growth factor (NGF)-differentiated PC12 cells exposed to ischemia by secretion of IL-6 and VEGF Human PLacental eXpanded (PLX) mesenchymal-like adherent stromal cells confer neuroprotection to nerve growth factor (NGF)-differentiated PC12 cells exposed to ischemia by secretion of IL-6 and VEGF](/preview/png/10802052.png)
چکیده انگلیسی
Mesenchymal stem cells are potent candidates in stroke therapy due to their ability to secrete protective anti-inflammatory cytokines and growth factors. We investigated the neuroprotective effects of human placental mesenchymal-like adherent stromal cells (PLX) using an established ischemic model of nerve growth factor (NGF)-differentiated pheochromocytoma PC12 cells exposed to oxygen and glucose deprivation (OGD) followed by reperfusion. Under optimal conditions, 2Â ÃÂ 105 PLX cells, added in a trans-well system, conferred 30-60% neuroprotection to PC12 cells subjected to ischemic insult. PC12 cell death, measured by LDH release, was reduced by PLX cells or by conditioned medium derived from PLX cells exposed to ischemia, suggesting the active release of factorial components. Since neuroprotection is a prominent function of the cytokine IL-6 and the angiogenic factor VEGF165, we measured their secretion using selective ELISA of the cells under ischemic or normoxic conditions. IL-6 and VEGF165 secretion by co-culture of PC12 and PLX cells was significantly higher under ischemic compared to normoxic conditions. Exogenous supplementation of 10Â ng/ml each of IL-6 and VEGF165 to insulted PC12 cells conferred neuroprotection, reminiscent of the neuroprotective effect of PLX cells or their conditioned medium. Growth factors as well as co-culture conditioned medium effects were reduced by 70% and 20% upon pretreatment with 240Â ng/ml Semaxanib (anti VEGF165) and/or 400Â ng/ml neutralizing anti IL-6 antibody, respectively. Therefore, PLX-induced neuroprotection in ischemic PC12 cells may be partially explained by IL-6 and VEGF165 secretion. These findings may also account for the therapeutic effects seen in clinical trials after treatment with these cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 2, February 2015, Pages 422-430
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 2, February 2015, Pages 422-430
نویسندگان
Adi Lahiani, Efrat Zahavi, Nir Netzer, Racheli Ofir, Lena Pinzur, Shani Raveh, Hadar Arien-Zakay, Ephraim Yavin, Philip Lazarovici,