کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10802280 | 1055679 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MAGI-1 acts as a scaffolding molecule for NGF receptor-mediated signaling pathway
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کلمات کلیدی
DMEMMAGI-1PTBPC12PDZNGFSHCPAGEERKGSTGFPPBSJnkFBSMBPc-Jun N-terminal kinase - C-Jun N-terminal kinasemembrane-associated guanylate kinase - guanylate kinase مرتبط با غشاءDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoRNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهMAGUK - آنهاphosphotyrosine binding - اتصال فسفاتیروسینpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدImmunoprecipitation - تخریب ایمنیTrk - تراکtropomyosin-related kinase - تریپومیوسین مرتبط با کینازfetal bovine serum - سرم جنین گاوnerve growth factor - فاکتور رشد عصبPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریgreen fluorescent protein - پروتئین فلورسنت سبزmaltose binding protein - پروتئین متصل به مالتوزglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have recently found that the membrane-associated guanylate kinase with inverted organization-1 (MAGI-1) was enriched in rat nervous tissues such as the glomeruli in olfactory bulb of adult rats and dorsal root entry zone in spinal cord of embryonic rats. In addition, we revealed the localization of MAGI-1 in the growth cone of the primary cultured rat dorsal root ganglion cells. These results point out the possibility that MAGI-1 is involved in the regulation of neurite extension or guidance. In this study, we attempted to reveal the physiological role(s) of MAGI-1 in neurite extension. We found that RNA interference (RNAi)-mediated knockdown of MAGI-1 caused inhibition of nerve growth factor (NGF)-induced neurite outgrowth in PC12 rat pheochromocytoma cells. To clarify the involvement of MAGI-1 in NGF-mediated signal pathway, we tried to identify binding partners for MAGI-1 and identified p75 neurotrophin receptor (p75NTR), a low affinity NGF receptor, and Shc, a phosphotyrosine-binding adaptor. These three proteins formed an immunocomplex in PC12 cells. Knockdown as well as overexpression of MAGI-1 caused suppression of NGF-stimulated activation of the Shc-ERK pathway, which is supposed to play important roles in neurite outgrowth of PC12 cells. These results indicate that MAGI-1 may act as a scaffolding molecule for NGF receptor-mediated signaling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1833, Issue 10, October 2013, Pages 2302-2310
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1833, Issue 10, October 2013, Pages 2302-2310
نویسندگان
Hidenori Ito, Rika Morishita, Ikuko Iwamoto, Makoto Mizuno, Koh-ichi Nagata,