کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10804516 | 1057280 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of conformational epitope of alginate-derived polymannuronates by surface plasmon resonance
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کلمات کلیدی
CSCHBS-EPFCMchondroitin sulfate CADPGOVAMonoclonal antibody - آنتی بادی مونوکلونالHyaluronic acid - اسید هیالورونیکOvalbumin - اوبلبومینEpitope - اپیتوپCSA - ایالات مؤتلفهٔ آمریکاSurface plasmon resonance - تشدید پلاسمون سطحیSPR - تشدید پلاسمون سطحیcircular dichroism - رنگ تابی دورانیBBB - سد خونی مغزیBlood–brain barrier - سد خونی مغزیDermatan sulfate - سولفات درماتانFlow cytometry - فلوسیتومتریHeparan sulfate - هپاران سولفاتchondroitin sulfate A - کندرویتین سولفات A
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the present study, we developed a mAb to alginate-derived polymannuronates (ADPM) and examined the antigenic epitopes using surface plasmon resonance (SPR) in conjunction with a large panel of oligomannuronate probes. We found that tetrasaccharide is the minimum-binding unit, and that increases in chain length from the tetrasaccharide to the heptsaccharide further enhance monovalent binding. A sharp increase in affinity was observed when increasing from the octasaccharide to the cosasaccharide, which is due to a further enhancement of the individual antigenic epitope combined with multivalency. Kinetic binding studies further suggested that the conformational epitope is discontinuous and infrequent and that a C6-carboxyl group is important in maintaining the conformational epitope. Moreover, CD analysis revealed there were conformational structures in epitopes. The data support our hypothesis that the conformational epitope for the mAb may be an extended helical segment of ADPM. ADPM exists mainly in linear form, but it can infrequently and spontaneously form extended helices. Although helices are not prevalent in ADPM, the immune system preferentially selects these conformational epitopes because they are unique. Together, our results indicate that the antigenic epitopes in β-d-mannuronates are conformational and require C6-carboxyl groups.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 87, Issue 11, November 2005, Pages 959-966
Journal: Biochimie - Volume 87, Issue 11, November 2005, Pages 959-966
نویسندگان
Li Gan, Xian L. Xin, Jing Li, Hai Y. Liu, Zhen Q. Zhang, Jin Qian, Jian Ding, Mei Y. Geng,