کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10814997 | 1058441 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
cAMP/PKA enhances interleukin-1β-induced interleukin-6 synthesis through STAT3 in glial cells
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کلمات کلیدی
GAPDHSTAT3pKaPGEJAK2CMIEpacSDSExchange proteins directly activated by cAMPDMEM8-bromo cAMPcAMP - cAMPDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoSAPK/JNK - SAPK / JNKSTAT - آمارstress-activated protein kinase/c-Jun N-terminal kinase - استنتاج پروتئین کیناز / c-Jun N-terminal kinaseinterleukin - اینترلوکینinterleukin-6 - اینترلوکین ۶Interleukin-1β - اینترلوکین-1βEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاCNS - دستگاه عصبی مرکزیsodium dodecyl sulfate - سدیم دودسیل سولفاتCytokine - سیتوکینcentral nervous system - سیستم عصبی مرکزیSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیmap - نقشهmitogen-activated protein - پروتئین فعال mitogenprotein kinase A - پروتئین کیناز Aprostaglandin - پروستاگلاندینهاJAK - چگونهglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We previously reported that interleukin (IL)-1β induces IL-6 synthesis via activation of the IκB/NFκB pathway, p38 mitogen-activated protein (MAP) kinase, stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), and signal transducer and activator of transcription (STAT)3, but not p44/p42 MAP kinase in rat glioma cell line, C6 cells and that cAMP enhances the IL-6 synthesis. However, the details behind enhancement of IL-1β-induced IL-6 synthesis by cAMP remain to be elucidated. In the present study, we investigated the exact mechanism of cAMP underlying the amplification of IL-1β-induced IL-6 synthesis in C6 cells. 8-Bromo cAMP significantly enhanced IL-1β-induced STAT3 phosphorylation without affecting phosphorylation of IκB, p38 MAP kinase or SAPK/JNK. In addition, we found that forskolin, a direct activator of adenylyl cyclase, significantly enhanced IL-1β-induced STAT3 phosphorylation. Janus family of tyrosine kinase (JAK) inhibitor I markedly suppressed the amplification by 8-bromo cAMP of IL-1β-induced IL-6 release. IL-1β induced JAK2 phosphorylation, and FLLL32, a specific JAK2 inhibitor, significantly reduced IL-1β-stimulated IL-6 release. 4-Cyano-3-methylisoquinoline, an inhibitor of protein kinase A (PKA), significantly attenuated the enhancing effect of 8-bromo cAMP on IL-1β-induced STAT3 phosphorylation. 8-Bromo cAMP markedly induced JAK2 phosphorylation. PKA siRNA transfection reduced enhancement of IL-1β-induced IL-6 release by 8-bromo cAMP. In conclusion, our results strongly suggest that the adenylyl cyclase/cAMP/PKA pathway upregulates IL-1β-induced IL-6 synthesis through enhancement of the JAK2/STAT3 pathway in C6 glioma cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 28, Issue 1, January 2016, Pages 19-24
Journal: Cellular Signalling - Volume 28, Issue 1, January 2016, Pages 19-24
نویسندگان
Kumiko Tanabe, Osamu Kozawa, Hiroki Iida,