کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815002 | 1058441 | 2016 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
FHL3 differentially regulates the expression of MyHC isoforms through interactions with MyoD and pCREB
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
MyogeninPGC-1αCREBpKaMyHCIGFsPVDMyoDFHLcyclic AMP-responsive elementperoxisome proliferator-activated receptor-γ coactivator-1αEMSACRESDScAMP-response-element-binding proteincDNA - cDNAComplementary DNA - DNA تکمیلیMRFs - MRF هاsmall hairpin RNA - RNA کوچک موی سرsiRNA - siRNAElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزchromatin immunoprecipitation - ایمن سازی کروماتینmyogenic differentiation 1 - تمایز ماهیانه 1coding sequence - توالی کدگذاریbase pair - جفت پایهMyosin heavy chain - زنجیره سنگین میوزینsodium dodecyl sulfate - سدیم دودسیل سولفاتinsulin-like growth factors - عوامل رشد انسولین مانندMYOG - منوMuscle fiber type - نوع فیبر عضلانیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازPolyvinylidene fluoride - پلی وینیلیدین فلورایدCHiP - چیپCdS - کادمیم سولفید، سولفید کادمیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In skeletal muscle, muscle fiber types are defined by four adult myosin heavy chain (MyHC) isoforms. Four and a half LIM domain protein 3 (FHL3) regulates myoblasts differentiation and gene expression by acting as a transcriptional co-activator or co-repressor. However, how FHL3 regulates MyHC expression is currently not clear. In this study, we found that FHL3 down-regulated the expression of MyHC 1/slow and up-regulated the expression of MyHC 2a and MyHC 2b, whereas no significant effect was found on MyHC 2x expression. MyoD and phosphorylated cAMP response element binding protein (pCREB) played important roles in the regulation of MyHC 1/slow and MyHC 2a expression by FHL3, respectively. FHL3 could interact with MyoD, CREB and pCREB in vivo. pCREB had stronger interaction with the cyclic AMP-responsive elements (CRE) of the MyHC 2a promoter compared with CREB, and FHL3 significantly affected the binding capacity of pCREB to CRE. We established a model in which FHL3 promotes the expression of MyHC 2a through CREB-mediated transcription and inhibits the expression of MyHC 1/slow by inhibiting MyoD transcription activity during myogenesis. Our data support the notion that FHL3 plays important roles in the regulation of muscle fiber type composition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 28, Issue 1, January 2016, Pages 60-73
Journal: Cellular Signalling - Volume 28, Issue 1, January 2016, Pages 60-73
نویسندگان
Yunxia Zhang, Wentao Li, Mingfei Zhu, Yuan Li, Zaiyan Xu, Bo Zuo,